Indicaxanthin from Cactus Pear Fruit Exerts Anti-Inflammatory Effects in Carrageenin-Induced Rat Pleurisy
Indicaxanthin from Cactus Pear Fruit Exerts Anti-Inflammatory Effects in Carrageenin-Induced Rat Pleurisy
复制标题
DOI：
10.3945/jn.113.183657
复制
发表时间：
2014-02-01
影响因子：
4.2
通讯作者：
Livrea, Maria Antonia
中科院分区：
文献类型：
--
作者：
Allegra, Mario;Ianaro, Angela;Livrea, Maria Antonia
营养研究最近从缓解营养缺乏转向慢性病预防。研究了食用仙人掌果实中甜菜色素类生物可利用的植物化学物质-仙人掌黄素的活性。米勒）在急性炎症的大鼠模型中。胸膜腔内注射0.2 mL卡拉胶制备大鼠胸膜炎模型，分别于4、24、48 h后处死大鼠，收集渗出液，测定一氧化氮（NO）、前列腺素E2（PGE 2）、白细胞介素1 β（IL-1 β）等炎症指标（IL-1 β）和肿瘤坏死因子-α（TNF-α）;分析胸膜中募集的细胞的环氧化酶-2（考克斯-2），诱导型一氧化氮合酶（iNOS）表达，和活化的B细胞（NF-κ B）活化的核因子κ轻链增强子。在角叉菜胶之前口服Indicaxanthin（0.5、1或2 μ mol/kg），呈时间和剂量依赖性，24 h时可减少渗出液体积（高达70%）和胸膜腔中募集的白细胞数量（高达95%）。2 μ mol/kg的Indixanthin预处理抑制角叉菜胶诱导的PGE释放（2）（91.4%），无（67.7%），IL-1 β（53.6%）和TNF-α（71.1%），并引起IL-1 β的降低（34.5%）、TNF-α（81.6%）、iNOS（75.2%）和COX-2（87.7%）mRNA以及iNOS（71.9%）和考克斯-2（65.9%）蛋白表达。Indicaxanthin抑制时间和剂量依赖性的NF-κ B的激活，在整个炎症级联的关键转录因子B。单次2 μ mol/kg口服给药的药代动力学研究显示，Indaxanthin的最大血浆浓度为0.22 +/-0.02 μ mol/L（n= 15），半衰期为1.15 +/- 0.11 h。当考虑到indixanthin在人类中的高生物利用度时，我们的研究结果表明，这种膳食色素具有改善健康和预防炎症性疾病的潜力。
Nutritional research has shifted recently from alleviating nutrient deficiencies to chronic disease prevention. We investigated the activity of indicaxanthin, a bioavailable phytochemical of the betalain class from the edible fruit of Opuntia ficus-indica (L. Miller) in a rat model of acute inflammation. Rat pleurisy was achieved by injection of 0.2 mL of lambda-carrageenin in the pleural cavity, and rats were killed 4, 24, and 48 h later; exudates were collected to analyze inflammatory parameters, such as nitric oxide (NO), prostaglandin E-2 (PGE(2)), interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha); cells recruited in pleura were analyzed for cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) expression, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) activation. Indicaxanthin (0.5, 1, or 2 mu mol/kg), given orally before carrageenin, time- and dose-dependently, reduced the exudate volume (up to 70%) and the number of leukocytes recruited in the pleural cavity (up to 95%) at 24 h. Pretreatment with indicaxanthin at 2 mu mol/kg inhibited the carrageenin-induced release of PGE(2) (91.4%), NO (67.7%), IL-1 beta (53.6%), and TNF-alpha (71.1%), and caused a decrease of IL-1 beta (34.5%), TNF-alpha (81.6%), iNOS (75.2%), and COX2 (87.7%) mRNA, as well as iNOS (71.9%) and COX-2 (65.9%) protein expression, in the recruited leukocytes. Indicaxanthin inhibited time- and dose-dependently the activation of NF-kappa B, a key transcription factor in the whole inflammatory cascade. A pharmacokinetic study with a single 2 mu mol/kg oral administration showed a maximum 0.22 +/- 0.02,mu mol/L (n= 15) plasma concentration of indicaxanthin, with a half-life of 1.15 +/- 0.11 h. When considering the high bioavailability of indicaxanthin in humans, our findings suggest that this dietary pigment has the potential to improve health and prevent inflammation-based disorders.