The elevated serum levels of calcineurin and nuclear factor of activated T-cells 1 in children with Kawasaki disease
The elevated serum levels of calcineurin and nuclear factor of activated T-cells 1 in children with Kawasaki disease
复制标题
川崎病患儿血清钙调神经磷酸酶和活化T细胞核因子1水平升高
DOI：
10.1186/s12969-020-0420-8
复制
发表时间：
2020-03-17
影响因子：
2.5
通讯作者：
Gong, Fangqi
中科院分区：
文献类型：
--
作者：
Sun, Yameng;Liu, Jingjing;Gong, Fangqi
研究背景钙调神经磷酸酶和活化T细胞核因子(CaN-NFAT)信号通路与川崎病(KD)易感性及冠状动脉动脉瘤形成密切相关。目的探讨川崎病(KD)患儿血清钙调神经磷酸酶(CaN)和活化T细胞核因子1(NFAT1)水平的变化。方法采用双抗体夹心酶联免疫吸附试验检测了66例健康儿童和74例KD患儿急性期、急性期和亚急性期血清CaN和NFAT1水平。结果急性期血清CaN和NFAT1水平显著升高，急性期和亚急性期逐渐下降，C反应蛋白、白细胞和中性粒细胞减少。在急性期、无热期和亚急性期，KD患者CaN和NFAT1的表达均显著高于健康对照组。IVIG治疗后，IVIG应答者血清CaN和NFAT1水平显著下降。然而，IVIG无应答者的CaN和NTAT1水平下降缓慢。在无热期，有冠状动脉病变的KD患者血浆NFAT1水平(268.82 ± 11.96 ng/ml)低于无冠状动脉病变的KD患者(285.84 ± 25.13 ng/ml)。而有CALS的KD患者与无CALS的KD患者血清CaN水平差异无统计学意义。结论KD患者血清CaN和NFAT1水平在KD发病过程中的特异性调节可能与KD的发生发展有关。
BackgroundThe calcineurin and nuclear factor of activated T-cells (CaN-NFAT) signaling pathway had been found to be associated with Kawasaki disease (KD) susceptibility and coronary artery aneurysm formation as a contributor. To evaluate serum calcineurin (CaN) and nuclear factor of activated T-cells 1(NFAT1) levels in patients with Kawasaki disease (KD).MethodsSerum levels of CaN and NFAT1 were measured by enzyme-linked immunosorbent assay method in 66 healthy children and 74 KD patients at acute, afebrile and subacute stage.ResultsThe serum levels of CaN and NFAT1 increased significantly in the acute stage, and decreased progressively in the afebrile and subacute stage, along with the reduction of C-reactive protein, white blood cells and neutrophil counts. And in the acute stage, the afebrile stage and the subacute stage, the expression of CaN and NFAT1 was upregulated significantly in KD patients compared to that in the healthy control. After the IVIG treatment, the serum levels of CaN and NFAT1 declined significantly in IVIG responders. However, the CaN and NTAT1 levels in the IVIG non-responders declined slowly. And in the afebrile stage, the NFAT1 levels were lower in KD patients with coronary artery lesions (CALs) (268.82 ± 11.96 ng/ml) than those without CALs (285.84 ± 25.13 ng/ml). However, the serum levels of CaN in KD patients with CALs had no significant difference with those in KD patients without CALs.ConclusionsThe specific regulation of CaN and NFAT1 serum levels in the course of KD was suggested that both of them were related in the development of KD.