Streptococcus uberis-specific T cells are present in mammary gland secretions of cows and can be activated to kill S. uberis
Streptococcus uberis-specific T cells are present in mammary gland secretions of cows and can be activated to kill S. uberis
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DOI：
10.1007/s11259-011-9462-1
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发表时间：
2011-03-01
影响因子：
2.2
通讯作者：
Wedlock, D. Neil
中科院分区：
文献类型：
--
作者：
Denis, Michel;Lacy-Hulbert, S. Jane;Wedlock, D. Neil
研究了奶牛乳房分泌物和血液中特异性T细胞的存在、表型和功能。评估了可持续性。将乳房中的MGS T细胞纯化并与自体血单核细胞一起孵育作为抗原呈递细胞（APC）。大多数奶牛，无论先前的S。哺乳期感染状况和泌乳状况，显示有S.在MGS和血液中的uberis特异性T细胞。当研究一组母牛的细胞时，发现S。uberis特异性T细胞产生高水平的干扰素-γ（IFN-γ），但低水平的白细胞介素-10（IL-10）。高百分比的应答T细胞是CD8（+）记忆（CD45RO）亚群。来自MGS的对S.在干乳期从动物中繁殖细胞，并使用白细胞介素-2（IL-2）和S.免疫球蛋白抗原这导致携带记忆细胞标志物（CD45A（-）、CD45RO（+））的CD8（+）亚群的T细胞的积累，其释放高水平的IFN-γ。从三只动物的MGS衍生的五种T细胞系中的四种对S.体外培养。结果表明，S.在感染或环境暴露于S.不稳定。旨在激活和扩增牛乳腺中的这种T细胞群的疫苗可能为预防由S.不稳定。
The presence, phenotype and function of Streptococcus uberis-specific T cells in the mammary gland secretion (MGS) and blood of cows exposed to S. uberis were assessed. MGS T cells in the udder were purified and incubated with autologous blood monocytes as antigen-presenting cells (APC). Most cows, irrespective of prior S. uberis infection status and lactation status, were shown to have S. uberis-specific T cells both in MGS and in the blood. When cells from a subgroup of cows were studied, it was found that the S. uberis-specific T cells produced high levels of interferon-gamma (IFN-gamma), but low levels of interleukin-10 (IL-10). A high percentage of responding T cells were of the CD8 (+) memory (CD45RO) subset. T cells from the MGS specific for S. uberis were propagated from animals during the drying off period and expanded in vitro using interleukin-2 (IL-2) and S. uberis antigens. This led to the accumulation of T cells of the CD8 (+) subset bearing memory cell markers (CD45A (-) , CD45RO (+) ), which released high levels of IFN-gamma. Four of the five T cell lines derived from the MGS of three animals had substantial direct killing activity towards S. uberis in vitro. It is concluded that there is an emergence of S. uberis-specific bactericidal T cells in the MGS of cows after infection or environmental exposure to S. uberis. Vaccines aimed at activating and expanding this T cell population in the mammary glands of cattle may offer an avenue for the prevention of mastitis caused by S. uberis.