Follicular fluid concentration of leukaemia inhibitory factor is decreased among women with polycystic ovarian syndrome during assisted reproduction cycles
Follicular fluid concentration of leukaemia inhibitory factor is decreased among women with polycystic ovarian syndrome during assisted reproduction cycles
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DOI：
10.1093/humrep/16.10.2073
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发表时间：
2001-10-01
影响因子：
6.1
通讯作者：
Chaouat, G
中科院分区：
文献类型：
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作者：
Lédée-Bataille, N;Laprée-Delage, G;Chaouat, G
背景：研究了多囊卵巢综合征（PCOS）患者卵巢中检测到特定细胞因子谱的可能性。方法：采用酶联免疫吸附试验（ELISA）或生物测定法对15例PCOS患者、15例周期正常的不育对照患者和8例卵母细胞供者卵巢刺激后收集的卵泡液中白血病抑制因子（LIF）、肿瘤坏死因子、白细胞介素11、γ干扰素、黄体酮和雌二醇的浓度进行测定。结果：PCOS患者卵泡液中LIF和孕酮浓度（LIF中位数：265 pg/ml）显著低于对照组（LIF中位数：816 pg/ml）；LIF与黄体酮卵泡液浓度相关（r = 0.720, P = 0.0001）。LH/FSH比值与LIF浓度呈负相关（r = -0.714, P = 0.0075）。尽管PCOS患者与对照组在年龄、卵巢储备、体外受精适应症等方面无明显差异，但PCOS患者的着床率明显低于对照组（IR = 9, P = < 0.01）。结论：卵泡液LIF具有促胚胎发育作用，PCOS患者的细胞因子谱可能与低着床率有关。
BACKGROUND: The possibility that a specific cytokine profile could be detected in the ovaries of patients with polycystic ovarian syndrome (PCOS) was investigated. METHOD: Enzyme-linked immunosorbent assay (ELISA) or bioassays were used to assess the concentrations of leukaemia inhibitory factor (LIF), tumour necrosis factor, interleukin 11, gamma interferon, progesterone and oestradiol in follicular fluids from preovulatory follicles collected after ovarian stimulation from 15 PCOS patients, 15 infertile control patients with regular cycles, and 8 oocyte donors. RESULTS: LIF and progesterone concentrations were significantly lower in the follicular fluid of PCOS patients (LIF median: 265 pg/ml) compared with controls (LIF median: 816 pg/ml); LIF and progesterone follicular fluid concentrations were correlated (r = 0.720, P = 0.0001). The LH/FSH ratio was negatively correlated with LIF concentrations (r = -0.714, P = 0.0075). Although the PCOS and control patients did not differ significantly in age, ovarian reserve or IVF indication, the implantation rate was significantly lower among the women with PCOS (IR = 9 versus 21%, P = < 0.01). CONCLUSION: The specific cytokine profile of the PCOS patients is probably related to the lower implantation rate since follicular fluid LIF appears to function as an embryotrophic agent.