Mouse models for tumor metastasis.
Mouse models for tumor metastasis.
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DOI：
10.1007/978-1-62703-008-3_17
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Huang, Xin-Yun
中科院分区：
文献类型：
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作者：
Yang, Shengyu;Zhang, J Jillian;Huang, Xin-Yun
肿瘤转移是癌症患者死亡的主要原因。在这里，我们描述了两种用于研究肿瘤转移的小鼠模型。在第一个小鼠自发转移模型中，将4T1小鼠乳腺肿瘤细胞注射到宿主小鼠的乳腺中，用结肠镜检测4T1肿瘤细胞向肺转移的情况。在第二个实验性转移小鼠模型中，将荧光素酶标记的MDA-MB-231人乳腺肿瘤细胞注射到NOD-SCID免疫缺陷小鼠的尾静脉中，使用无创生物发光成像监测MDA-MB-231肿瘤细胞在肺部的定植。
Tumor metastasis is the main cause of death of cancer patients. Here we describe two mouse models for investigating tumor metastasis. In the first spontaneous metastasis mouse model, 4T1 mouse breast tumor cells are injected into the mammary gland of host mice and the metastasis of 4T1 tumor cells into the lung are examined with a colonogenic assay. In the second experimental metastasis mouse model, luciferase-labeled MDA-MB-231 human breast tumor cells are injected into the tail vein of NOD-SCID immunodeficient mice and the colonization of MDA-MB-231 tumor cells in the lung are monitored using noninvasive bioluminescence imaging.