Identification of a sex-determining region in Nile tilapia (Oreochromis niloticus) using bulked segregant analysis
Identification of a sex-determining region in Nile tilapia (Oreochromis niloticus) using bulked segregant analysis
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DOI：
10.1046/j.1365-2052.2003.01035.x
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发表时间：
2003-10-01
期刊：
影响因子：
2.4
通讯作者：
Kocher, TD
中科院分区：
文献类型：
--
作者：
Lee, BY;Penman, DJ;Kocher, TD
尼罗罗非鱼的性别决定是由一对主基因控制的XX-XY系统。我们寻找DNA标记与这个主要位点使用散装分离分析。10个微卫星标记属于连锁群8被发现与表型性别。推测的Y染色体等位基因正确地预测了两个家庭中95%的男性和女性个体的性别。我们的研究结果表明，一个主要的性别决定位点内的几个厘标记UNH995和UNH104。来自同一人群的第三个家庭没有证据表明该区域与表型性别的联系，表明在一些家庭中，额外的遗传和/或环境因素调节性别决定。这些标记可直接用于研究不同Y染色体等位基因的强度，并用于鉴定携带一个或多个Y单倍型拷贝的亲鱼。
Sex determination in the Nile tilapia (Oreochromis niloticus) is thought to be an XX-XY (male heterogametic) system controlled by a major gene. We searched for DNA markers linked to this major locus using bulked segregant analysis. Ten microsatellite markers belonging to linkage group 8 were found to be linked to phenotypic sex. The putative Y-chromosome alleles correctly predict the sex of 95% of male and female individuals in two families. Our results suggest a major sex-determining locus within a few centimorgans of markers UNH995 and UNH104. A third family from the same population showed no evidence for linkage of this region with phenotypic sex, indicating that additional genetic and/or environmental factors regulate sex determination in some families. These markers have immediate utility for studying the strength of different Y chromosome alleles, and for identifying broodstock carrying one or more copies of the Y haplotype.