The ISWI chromatin remodelling factor NURF is not required for mitotic male X chromosome organisation.
The ISWI chromatin remodelling factor NURF is not required for mitotic male X chromosome organisation.
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DOI：
10.17912/micropub.biology.000360
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发表时间：
2021-01-26
影响因子：
--
通讯作者：
Badenhorst P
中科院分区：
文献类型：
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作者：
Kwon SY;Jang B;Badenhorst P
核小体重塑因子（NURF）是一种iswi类atp依赖性染色质重塑酶，是基因表达和高阶染色质组织所必需的。NURF与修饰果蝇多烯雄性X染色体的组蛋白修饰结合，是维持该染色体正确组织所必需的。NURF突变体表现出扭曲和去致密的多烯男性X染色体依赖于男性特异性致死（MSL）复合物的存在。在这里，我们测试了有丝分裂染色体是否同样需要NURF来维持正确的形态。令人惊讶的是，尽管MSL复合体仍然与有丝分裂的男性X染色体有关，但NURF并不需要维持形态。虽然已知NURF的ISWI亚基仍然与有丝分裂染色体相关，但我们发现NURF特异性亚基Nurf301/BPTF在果蝇和人类有丝分裂过程中都与染色质分离，进一步阐明了NURF在有丝分裂染色体组织中是必不可少的。
The nucleosome remodelling factor (NURF) is an ISWI-class ATP-dependent chromatin remodeling enzyme required both for gene expression and higher order chromatin organisation. NURF binds to histone modifications that decorate the Drosophila polytene male X chromosome and is required to maintain correct organisation of this chromosome. NURF mutants exhibit distorted and decondensed polytene male X chromosomes dependent on the presence of the male-specific lethal (MSL) complex. Here we tested whether mitotic chromosomes similarly require NURF to maintain correct morphology. Surprisingly, although the MSL complex remains associated with mitotic male X chromosomes, NURF is not required to maintain morphology. While the ISWI subunit of NURF is known to remain associated with mitotic chromosomes we show that the NURF specificity subunit Nurf301/BPTF dissociates from chromatin during both Drosophila and human mitosis, further illuminating that NURF is dispensable for mitotic chromosome organisation.