PARAMETERS OF UN-BALANCED GROWTH AND REVERSIBLE INHIBITION OF DEOXYRIBONUCLEIC-ACID SYNTHESIS IN BREVIBACTERIUM-AMMONIAGENES ATCC 6872 INDUCED BY DEPLETION OF MN2+ - INHIBITOR STUDIES ON THE REVERSIBILITY OF DEOXYRIBONUCLEIC-ACID SYNTHESIS
PARAMETERS OF UN-BALANCED GROWTH AND REVERSIBLE INHIBITION OF DEOXYRIBONUCLEIC-ACID SYNTHESIS IN BREVIBACTERIUM-AMMONIAGENES ATCC 6872 INDUCED BY DEPLETION OF MN2+ - INHIBITOR STUDIES ON THE REVERSIBILITY OF DEOXYRIBONUCLEIC-ACID SYNTHESIS
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DOI：
10.1007/bf00428013
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发表时间：
1980-01-01
影响因子：
2.8
通讯作者：
DIEKMANN, H
中科院分区：
文献类型：
--
作者：
AULING, G;THALER, M;DIEKMANN, H
Unbalanced growth induced by depletion of Mn2+ was a prerequisite for production of ribonucleotides in a high salt mineral medium with the wildtype strain B. ammoniagenes ATCC 6872. The concentration of Mn strictly controlled the overall DNA synthesis, whereas RNA, protein and cell wall synthesis remained essentially unimpaired in the Mn-lacking cells. The reversibility of inhibition of overall DNA synthesis was shown by enhanced incorporation (up to 3-fold compared to the cultures supplied with sufficient Mn) of [8-14C]adenine into alkali-stable, trichloroacetic acid-insoluble material after subsequent addition of 10 .mu.M MnCl2 to 15-h-old depleted cultures. The results of inhibitor studies on the restoration of overall DNA synthesis due to subsequent addition of Mn2+ to depleted cultures suggest that ribonucleotide reduction is the primary target of the Mn starvation during nucleotide fermentation with B. ammoniagenes ATCC 6872.
