Optimization of integrated extraction-adsorption process for the extraction and purification of total flavonoids from Scutellariae barbatae herba
Optimization of integrated extraction-adsorption process for the extraction and purification of total flavonoids from Scutellariae barbatae herba
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DOI：
10.1016/j.seppur.2016.11.031
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发表时间：
2017-03-24
影响因子：
8.6
通讯作者：
Wu, Yongjiang
中科院分区：
文献类型：
--
作者：
Huang, Peng;Zhang, Qing;Wu, Yongjiang
采用萃取-吸附一体化工艺对半枝莲总黄酮进行提取纯化。通过大孔吸附树脂筛选、单因素试验、Box-Behnken设计试验和纯化工艺条件研究，优化了大孔吸附树脂的纯化工艺。以DM 130树脂为吸附剂，确定了最佳萃取-吸附条件为：萃取剂pH 2，萃取时间9 h，液固比20：1 mL/g，流速7 BV/h。选择70%（5 BV）乙醇水溶液作为洗脱溶剂。该工艺以水为提取溶剂，可完全提取出半枝莲中的总黄酮。该集成工艺简化了操作，降低了能耗，防止了生物活性物质的降解，提高了产品质量。与传统方法相比，该方法总黄酮的收率和纯度分别提高了1.50倍和1.16倍。结果表明，该集成工艺高效、环保，适合于大规模提取和纯化半枝莲总黄酮。(C)© 2016 Elsevier B. V.版权所有。
In the present study, integrated extraction-adsorption process was developed and applied to extract and purify total flavonoids from Scutellariae barbatae herba. Screening of macroporous resins, single factor experiments, an experimental design based on Box-Behnken design (BBD) and purification conditions study were carried out to optimize the integrated process. DM130 resin was chosen as adsorbent and the optimal extraction-adsorption conditions were determined as pH of extraction solvent 2, time 9 h, liquid/solid ratio 20:1 mL/g and flow rate 7 BV/h. 70% (5 BV) ethanol aqueous solution was selected as elution solvent. Through this procedure, the total flavonoids of Scutellariae barbatae herba can be totally extracted using water as extraction solvent rather than high concentration ethanol was used in traditional extraction method. Moreover, the integrated process can simplify operation, reduce energy consumption, and prevent the degradation of bioactive substances, thus improve the quality of products. Compared to the conventional method, the recovery yield and purity of total flavonoids obtained from this procedure increased by 1.50 and 1.16 times respectively. The results showed that the integrated procedure can be efficient and eco-friendly, as well as appropriate for large-scale extraction and purification of total flavonoids from Scutellariae barbatae herba. (C) 2016 Elsevier B.V. All rights reserved.