Biocatalytic asymmetric hydroxylation of hydrocarbons by free and immobilized Bacillus megaterium cells
Biocatalytic asymmetric hydroxylation of hydrocarbons by free and immobilized Bacillus megaterium cells
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DOI：
10.1016/s1381-1177(00)00028-x
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发表时间：
2001-01-22
影响因子：
--
通讯作者：
Schreier, P
中科院分区：
文献类型：
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作者：
Adam, W;Lukacs, Z;Schreier, P
用烯丙基苯筛选土壤细菌，产生了巨大芽孢杆菌菌株，该菌株可羟基化高对映体过量的简单碳氢化合物（高达 99%）。获得了苄基和非苄基羟基化产物，没有通常观察到的对苄基位置的高度偏好。将巨大芽孢杆菌细胞固定在藻酸盐凝胶中，有效提高了细胞的稳定性，增加了形成的产物的量，且不损失对映选择性。产物比例（α 与 β 羟基化）转向苄基羟基化，这表明至少涉及两种具有不同区域选择性的羟基化酶。与小型和大型生物反应器（高达 2000 mi）中的自由细胞发酵的比较表明，使用固定化细胞是有利的，因为它们更容易处理并产生更多的氧化产物。 (C) 2001 Elsevier Science B.V. 保留所有权利。
Screening of soil bacteria with allylbenzene resulted in a Bacillus megaterium strain, which hydroxylates simple hydrocarbons in high enantiomeric excess tee up to 99%). Benzylic and nonbenzylic hydroxylation products were obtained, without the usually observed high preference for the benzylic position. The immobilization of the B. megaterium cells in alginate gel effectively improved the stability of the cells and increased the amounts of products formed, without loss of enantioselectivity. The product ratio (alpha vs. beta hydroxylation) was shifted towards benzylic hydroxylation, which suggests that at least two hydroxylating enzymes with distinct regioselectivity are involved. Comparison to free-cell fermentations in small- and large-scale bioreactors (up to 2000 mi) showed that the use of immobilized cells is advantageous, as they are easier to handle and yield higher amounts of oxidation products. (C) 2001 Elsevier Science B.V. All rights reserved.