Metabolic biomarkers for monitoring anaerobic naphthalene biodegradation in situ.
Metabolic biomarkers for monitoring anaerobic naphthalene biodegradation in situ.
复制标题
用于监测厌氧萘原位生物降解的代谢生物标志物。
作者：
C. Phelps;J. Battistelli;L. Young
在萘和甲基萘的厌氧生物降解过程中，通过特定的微生物羧化和环还原反应形成独特的代谢产物。从缺氧，浅层含水层污染的汽油地下水样品进行了检查，这些代谢物的存在下，通过提取，衍生化和气相色谱-质谱联用。在地下水样品中发现了几种代谢物[2-萘甲酸（2-NA），四氢-2-萘甲酸（TH-2-NA），六氢-2-萘甲酸（HH-2-NA）和甲基萘甲酸（MNA）]。在每个监测孔的2-NA的浓度进行定量和相关的污染区。在与2-NA相同的威尔斯孔中存在其他代谢物，用于确认厌氧途径确实具有活性。代谢物在该站点的分布表明，它们可以用作生物标志物，用于证明原位生物降解。
During the anaerobic biodegradation of naphthalene and methylnaphthalene, unique metabolites are formed by specific microbial carboxylation and ring-reduction reactions. Groundwater samples from an anoxic, shallow aquifer contaminated with gasoline were examined for the presence of these metabolites by extraction, derivatization and gas chromatography coupled to mass spectroscopy. Several metabolites [2-naphthoic acid (2-NA), tetrahydro-2-naphthoic acid (TH-2-NA), hexahydro-2-naphthoic acid (HH-2-NA) and methylnaphthoic acid (MNA)] were found to be present in the groundwater samples. The concentration of 2-NA at each monitoring well was quantified and correlated to the zones of contamination. The presence of the other metabolites in the same wells as 2-NA was used as confirmation that the anaerobic pathway was indeed active. The distribution of metabolites at this site shows that they can be used as biomarkers for demonstrating in situ biodegradation.