MX GENES SHOW WEAKER PRIMARY RESPONSE TO VIRUS THAN OTHER INTERFERON-REGULATED GENES
MX GENES SHOW WEAKER PRIMARY RESPONSE TO VIRUS THAN OTHER INTERFERON-REGULATED GENES
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DOI：
10.1016/0042-6822(92)90069-2
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发表时间：
1992-01-01
期刊：
影响因子：
3.7
通讯作者：
STAEHELI, P
中科院分区：
文献类型：
--
作者：
BAZZIGHER, L;PAVLOVIC, J;STAEHELI, P
一些受干扰素调节的基因既是对病毒的初级反应，也是通过病毒诱导的干扰素诱导的次要反应。在这里，我们研究了这种双重控制机制是否也会调节编码具有内在抗病毒潜力的蛋白质的人和鼠Mx基因的活性。为了区分对病毒的初次反应和对病毒诱导的干扰素的继发性反应，我们研究了病毒诱导的Mx基因在缺乏功能性干扰素系统的细胞系和蛋白质合成受阻的细胞中的表达。与干扰素调节的两个人类基因ISG56和ISG15不同，人MxA基因对新城疫病毒(NDV)或流感病毒几乎没有主要反应。同样，新城疫病毒或流感病毒直接激活小鼠Mx1基因在小鼠胚胎细胞或移植的腹膜巨噬细胞中并不显著。永久细胞系L1210对新城疫病毒有中等的原代Mx1应答。缺乏对病毒的强烈的非干扰素依赖的Mx反应，表明这种基因调控模式在Mx介导的对病毒疾病的抗性中不起重要作用。
Some interferon (IFN)-regulated genes are induced as a primary response to virus as well as secondarily through virus-induced IFN. Here we investigated whether this dual control mechanism would also regulate the activity of the human and mouseMxgenes that encode proteins with intrinsic antiviral potentials. To distinguish between a primary response to virus and a secondary response to virus-induced IFN, we studied virus-inducedMxgene expression in cell lines that lack a functional IFN system and in cells with blocked protein synthesis. In contrast to the two IFN-regulated human genesISG56andISG15, the humanMxAgene showed almost no primary response to Newcastle disease virus (NDV) or influenza virus. Similarly, direct activation of the mouseMx1gene by NDV or influenza virus was not significant in mouse embryo cells or explanted peritoneal macrophages. A moderate primaryMx1response to NDV was observed in the permanent cell line L1210. Lack of a strong IFN-independentMxresponse to virus indicates that this mode of gene regulation does not play a significant role inMx-mediated resistance to viral disease.