Impedance sensing and molecular modeling of an olfactory biosensor based on chemosensory proteins of honeybee
Impedance sensing and molecular modeling of an olfactory biosensor based on chemosensory proteins of honeybee
复制标题
基于蜜蜂化学感应蛋白的嗅觉生物传感器的阻抗传感和分子建模
DOI：
10.1016/j.bios.2012.07.011
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发表时间：
2013-02-15
影响因子：
12.6
通讯作者：
Wang, Ping
中科院分区：
文献类型：
--
作者：
Liu, Qingjun;Wang, Hua;Wang, Ping
通过模拟生物嗅觉，生物传感器已被用于检测复杂环境中的重要配体。将蜜蜂化学感受蛋白（Ac-ASP 3）固定在叉指型金电极上，设计了一种基于化学感受蛋白的嗅觉传感器。用阻抗谱法记录了它对信息素配体和花香的响应。从10（-7）M到10（-3）M，生物传感器的电荷转移电阻的相对降低与配体浓度的对数成正比。为了探索生物传感器的分子识别过程，对蛋白质的三级结构进行了建模，并通过分子对接研究了蛋白质与配体的相互作用。对接结果验证了实验的有效性，并表明特异性配体可以与Ac-ASP 3的Cys 60和Gln 64等保守残基形成氢键。此外，将分子模拟与阻抗检测相结合，可以提高配体与蛋白质结合的准确性、特异性和可预测性。因此，CSP将提供一个有前途的方法，在低浓度的化学分子传感。(C)2012爱思唯尔有限公司版权所有。
By mimicking biological olfaction, biosensors have been used for the detection of important ligands in complex environments. An olfactory biosensor based on chemosensory proteins (CSPs) was designed by immobilizing honeybee CSPs (Ac-ASP3) on the interdigitated golden electrodes. Its responses to ligands of pheromones and floral odors were recorded by impedance spectroscopy. The relative decrease of charge transfer resistance of the biosensor is proportional to the logarithm of ligand concentration from 10(-7) M to 10(-3) M. To explore the molecular recognition processes of the biosensor, the tertiary structure of the protein was modeled and the protein-ligand interactions were investigated by the molecular docking. Our docking results verified the validity of experiments and showed that the specific ligands could form hydrogen bonds with some of the conserved residues, such as Cys 60 and Gln 64 of Ac-ASP3. Furthermore, combining the molecular modeling with impedance detection, the accuracy, specificity and predictability of the ligands binding to the protein could be improved. Thus, CSPs will provide a promising approach for chemical molecular sensing at low concentrations. (C) 2012 Elsevier B.V. All rights reserved.