Ion sensing film optodes: disposable ion sensing probes for the determination of Na+, K+, Ca2+ and Cl− concentrations in serum
Ion sensing film optodes: disposable ion sensing probes for the determination of Na+, K+, Ca2+ and Cl− concentrations in serum
复制标题
离子传感薄膜光极：用于测定血清中 Na+、K+、Ca2+ 和 Cl− 浓度的一次性离子传感探针
DOI：
10.1016/0925-4005(95)01711-9
复制
发表时间：
1995
影响因子：
8.4
通讯作者：
Koji Suzuki
中科院分区：
文献类型：
--
作者：
H. Hisamoto;Nami Miyashita;Kazuhiko Watanabe;Eriko Nakagawa;N. Yamamoto;Koji Suzuki
用一种新制备的亲脂性阴离子染料和含有相应离子载体的增塑PVC膜制备了用于测定Na+、K+、Ca ~（2+）、Cl ~-4种离子的高选择性离子敏感膜光电极。这些传感膜的光学响应的原理是基于离子-离子载体复合物和去质子化或质子化的亲脂性阴离子染料的离子对提取。这些膜光极是一次性使用的，并且可以通过简单地将它们浸入填充有样品溶液的玻璃容器中并通过测量在可见光区中亲脂性阴离子染料的相应最大吸收波长处的吸光度变化来确定离子来检测离子。基于离子引出模型对薄膜的理论响应进行了讨论。这些膜的离子选择性足以测定稀释血清样品中的Na+、K+、Ca 2+和Cl−，在血清分析中获得了令人满意的结果。
Highly selective ion sensing film optodes for the determination of four kinds of ions (Na+, K+, Ca2+, Cl−) were prepared with plasticized PVC membranes containing the respective ionophores and a newly prepared lipophilic anionic dye. The principle of the optical response of these sensing films is based on ion-pair extraction of the ion-ionophore complex and a deprotonated or protonated lipophilic anionic dye. These film optodes are for disposable use and can detect ions by simply immersing them into a glass vessel which is filled with the samples solution and determining the ion by measuring the absorbance change at the respective maximum absorption wavelength of the lipophilic anionic dyes in the visible region. The theoretical response of these films was also discussed based on the ion-extraction model. The ion selectivity of these films was sufficient for the determination of Na+, K+, Ca2+and Cl−in diluted serum samples, and satisfactory results were obtained in serum analysis.