Cutting edge:: Human Th17 cells are identified as bearing CCR2+CCR5- phenotype
Cutting edge:: Human Th17 cells are identified as bearing CCR2+CCR5- phenotype
复制标题
DOI：
10.4049/jimmunol.178.12.7525
复制
发表时间：
2007-06-15
影响因子：
4.4
通讯作者：
Yamamura, Takashi
中科院分区：
文献类型：
--
作者：
Sato, Wakiro;Aranami, Toshimasa;Yamamura, Takashi
最近的报道表明，产生il -17的CD4(+) T细胞（Th17细胞）属于一种独特的辅助性T细胞谱系，并在自身免疫性疾病和过敏的发病过程中起关键作用。然而，Th17细胞的趋化因子受体谱尚不清楚。在本研究中，我们报道了人类Th17细胞被鉴定为CCR2(+) CCR5（-）记忆CD4(+) T细胞。对健康供体PBMC的分析表明，CCR2（+）细胞比CCR2（-）细胞产生更多的IL-17，表明CCR2在Th17细胞上优先表达。值得注意的是，CCR2+ CCR5-记忆CD4+ T细胞产生大量的IL-17和少量的ifn - γ，而CCR2(+) CCR5（+）细胞反过来产生大量的ifn - γ而少量的IL-17。此外，T-bet在CCR5+记忆T细胞中表达较高。这些结果表明，缺乏CCR5可以区分人类Th17细胞和Th1细胞。
Recent reports have shown that IL-17-producing CD4(+) T cells (Th17 cells) belong to a distinct helper T cell lineage and are critically involved in the pathogenesis of autoim- mune diseases and allergies. However, the chemokine receptor profile of Th17 cells remains to be clarified In this study, we report that human Th17 cells are identified as CCR2(+) CCR5(-) memory CD4(+) T cells. Analysis of PBMC from healthy donors showed that CCR2(+) cellsproduce much larger amounts of IL-17 than CCR2(-) cells, indicating the preferential expression of CCR2 on Th17 cells. Notably, CCR2+ CCR5- memory CD4+ T cells produced a large amount of IL-17 and little IFN-gamma, whereas CCR2(+) CCR5(+) cells reciprocally produced an enormous amount of IFN-gamma but little IL-17. Moreover, a higher expression of T-bet was seen in the CCR5+ memory T cells. These results indicate that absence of CCR5 distinguishes human Th17 cells from Th1 cells.