Conditional siphon priming for multi-step assays on centrifugal microfluidic platforms
Conditional siphon priming for multi-step assays on centrifugal microfluidic platforms
复制标题
用于离心微流控平台上多步分析的条件虹吸启动
DOI：
10.1016/j.snb.2016.11.063
复制
发表时间：
2017
影响因子：
8.4
通讯作者：
Cheng Jing
中科院分区：
文献类型：
--
作者：
Meng Xiangrui;Zhu Yunzeng;Chen Yiqi;Lu Ying;Xu Youchun;Cheng Jing
离心微流体已被证明在生物医学诊断、生物分析和环境监测方面是成功的。然而，多步骤样品处理、反应和检测的自动化仍然是一个巨大的挑战。在本研究中，介绍了一种条件虹吸引液技术，用于多步液体添加或选择性路由。由于虹吸通道被局部修改或通风被另一个腔室的液体阻塞，虹吸启动可以通过添加液体或低频通风来触发。使用该技术，成功地实现了液体的顺序释放和多种方式的选择性路由。为了验证这一概念，设计了一个离心微流控平台，用于水样的现场铵分析。铵浓度的线性范围通过稀释过程的整合得到扩展。这种新颖的阀门技术为离心平台上复杂的液体处理过程的集成提供了新的解决方案。
Centrifugal microfluidics has proven to be successful in biomedical diagnostics, biological analysis and environmental monitoring. However, the automation of multi-step sample processing, reaction and detection remains a great challenge. In this study, a conditional siphon priming technique is introduced for multi-step liquid addition or selective routing. Since the siphon channel is locally modified or venting is blocked by liquid in another chamber, siphon priming can be triggered by liquid addition or venting at low frequency. Using this technique, sequential release of liquids and selective routing in multiple manners were successfully achieved. As a proof of concept, a centrifugal microfluidic platform was designed for on-site ammonium analysis in water samples. The linear range of ammonium concentrations is extended by integration of a dilution process. This novel valving technique provides new solutions for integration of complex liquid handling processes on centrifugal platforms.