Amphotericin B tissue distribution in autopsy material after treatment with liposomal amphotericin B and amphotericin B colloidal dispersion
Amphotericin B tissue distribution in autopsy material after treatment with liposomal amphotericin B and amphotericin B colloidal dispersion
复制标题
DOI：
10.1093/jac/dkl141
复制
发表时间：
2006-06-01
影响因子：
5.2
通讯作者：
Bellmann, Romuald
中科院分区：
文献类型：
--
作者：
Vogelsinger, Helene;Weiler, Stefan;Bellmann, Romuald
目的：在接受脂质体阿替霉素B或阿替霉素B胶体分散体治疗的患者的尸检材料中测定阿替霉素B的组织浓度（胶体阿替霉素B）用于疑似或证实的侵袭性真菌感染。对20例接受脂质制剂两性霉素B治疗的患者的肝、脾、肺、肾和心肌组织中的两性霉素B组织水平进行了测定，在他们死于多器官衰竭之前7名患者接受脂质体阿替西汀B（AmBisome（R））治疗，13名患者接受胶体阿替西汀B（Amphotericin（R））治疗。在常规尸检期间获得组织样品，均质化并用甲醇提取。结果：两性霉素B在肝脏和脾脏中的含量最高，其次是肾脏、肺、心肌和脑。在肺中发现更高的阿替霉素B浓度后，治疗与阿替霉素B胶体分散体比治疗后与脂质体阿替霉素B。结论：脂质制剂的选择可能会影响阿替霉素B渗透到肺。
Objectives: Tissue concentrations of amphotericin B were determined in autopsy material of patients who had been treated with liposomal amphotericin B or amphotericin B colloidal dispersion (colloidal amphotericin B) for suspected or proven invasive fungal infection.Patients and methods: Amphotericin B tissue levels were measured in liver, spleen, lung, kidney, and myocardial find brain tissue of 20 patients who had been treated with lipid-formulated amphotericin B, before they died from multi-organ failure. Seven patients had been treated with liposomal amphotericin B (AmBisome (R)) and thirteen with colloidal amphotericin B (Amphocil (R)). Tissue samples were obtained during routine autopsy, homogenized and extracted with methanol. Amphotericin B concentrations were measured using HPLC after purification by solid phase extraction.Results: The highest amphotericin B levels were found in liver and spleen, followed by kidney, lung, myocardium and brain. In the lung higher amphotericin B concentrations were found after treatment with amphotericin B colloidal dispersion than after therapy with liposomal amphotericin B.Conclusions: The choice of lipid formulation may influence amphotericin B penetration into the lung.