Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles
Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles
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DOI：
10.1016/j.etap.2010.05.004
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发表时间：
2010-09-01
影响因子：
4.3
通讯作者：
Park, Kwangsik
中科院分区：
文献类型：
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作者：
Park, Eun-Jung;Bae, Eunjoo;Park, Kwangsik
纳米颗粒的毒性取决于许多因素，包括尺寸、形状、化学组成、表面积、表面电荷。等人在这项研究中，我们比较了不同尺寸的银纳米颗粒（AgNPs）的毒性，这些银纳米颗粒因其独特的抗菌活性而广泛用于消费品中。结果表明，在脑、肺、肝、肾、睾丸等组织中均未检测到22 nm、42 nm和71 nm的AgNPs，而在这些组织中未检测到323 nm的AgNPs。血清中的TGF-β水平在小尺寸AgNP的处理组中也显著增加，而在大尺寸AgNP中没有。此外，通过表型分析，B细胞分布在小尺寸AgNP中增加，而在大尺寸AgNP中没有。体重或器官/体重比在对照组和所有AgNPs处理组之间没有差异。AgNPs的重复剂量毒性（42 nm）的荧光强度，并在小鼠中进行了28天的口服给药研究（0.25mg/kg、050 mg/kg、100 mg/kg），高剂量组对肝脏、肾脏有不良影响（100 mg/kg），当通过血液化学和组织病理学分析测定时，细胞因子包括IL-1、IL-6、IL-4、IL-10、IL-12。此外，淋巴细胞中的B细胞分布和IgE产生增加。基于这些结果，提示重复口服施用纳米尺寸的AgNP可能在小鼠中引起器官毒性和炎症反应（C）2010 Elsevier B V保留所有权利。
Toxicity of nanoparticles depends on many factors including size, shape, chemical composition, surface area,surface charge. and others. In this study, we compared the toxicity of different sized-silver nanopartcles (AgNPs) which are being widely used in consumer products clue to its unique antimicrobial activity When mice were treated with AgNPs 1 mg/kg for 14 days by oral administration, small-sized AgNPs (22 nm, 42 nm, and 71 nm) were distributed to the organs including brain, lung, liver, kidney, and testis while large-sized AgNPs (323 nm) were not detected in those tissues. The levels of TGF-beta in serum were also significantly increased in the treated group of small-sized AgNPs but not in large-sized AgNPs In addition, B cell distribution was Increased in small-sized AgNPs but not in large-sized-AgNPs by the phenotype analysis However, body weight or in the ratio of organ/body weight were not different between the control group and all the AgNPs-treated groups The repeated-dose toxicity of AgNPs (42 nm) was also investigated in mice by oral administration for 28 clays By the administration of AgNPs (0.25 mg/kg, 050 mg/kg, 1 00 mg/kg), adverse impacts on liver and kidney were observed in a high dose-treated group (1 00 mg/kg), when determined by blood chemistry and histipathological analysis Cytokines including IL-1, IL-6, IL-4, IL-10, IL-12. and TGF-beta were also increased in a dose-dependent manner by repeated oral administration In addition, B cell distribution in lymphocyte and IgE production were increased Based on these results, it is suggested that repeated oral administration of nano-sized AgNPs may cause organ toxicity and inflammatory responses in mice (C) 2010 Elsevier B V All rights reserved.