Stem cell factor regulates human melanocyte-matrix interactions.
Stem cell factor regulates human melanocyte-matrix interactions.
复制标题
干细胞因子调节人类黑素细胞-基质相互作用。
DOI：
10.1111/j.1600-0749.1994.tb00017.x
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Abboud,C
中科院分区：
文献类型：
--
作者：
Scott,G;Ewing,J;Ryan,D;Abboud,C
假设干细胞因子（SCF）在胚胎发生期间的黑素细胞迁移中起关键作用，因为SCF基因或其配体c-kit的突变导致小鼠的皮毛色素沉着和人类的皮肤色素沉着缺陷。在这份报告中，我们直接表明SCF改变了人类黑素细胞与细胞外基质（ECM）配体的粘附和迁移，并在蛋白水平调节整合素表达。SCF降低新生儿和胎儿细胞与IV型胶原的粘附，增加胎儿细胞与层粘连蛋白的粘附。胎儿细胞与纤连蛋白的粘附减少，但新生儿细胞中无变化。新生儿黑素细胞的流式细胞术分析显示，SCF下调α2受体的表达，上调α3、α5和β1整合素受体的表达。SCF下调胎儿黑素细胞α2、α5和β1整合素的表达，上调αv和α3整合素受体的表达。使用延时视频显微镜分析黑素细胞迁移显示，SCF显著增加新生儿黑素细胞在纤维连接蛋白（FN）上的迁移，但不增加胎儿黑素细胞的迁移。我们得出结论，SCF调节整合素的表达在蛋白质水平上，SCF对黑素细胞的附着和迁移ECM配体具有多效性。我们认为这可能是SCF在皮肤发育过程中调节黑素细胞迁移的一种机制。
Stem cell factor (SCF) is hypothesized to play a critical role in the migration of melanocytes during embryogenesis because mutations in either the SCF gene, or its ligand, c‐kit, result in defects in coat pigmentation in mice and in skin pigmentation in humans. In this report we directly show that SCF alters the adhesion and migration of human melanocytes to extracellular matrix (ECM) ligands and regulates integrin expression at the protein level. SCF decreased adhesion of neonatal and fetal cells to collagen IV, and increased attachment of fetal cells to laminin. Attachment of fetal cells to fibronectin was decreased, but was unchanged in neonatal cells. Flow cytometry analysis of neonatal melanocytes showed that SCF down‐regulated the expression of the α2 receptor, and up‐regulated the expression of the α3, α5 and β1 integrin receptors. SCF down‐regulated expression of α2, α5 and β1 integrins by fetal melanocytes, and up‐regulated expression of the αv and α3 integrin receptors. Analysis of melanocyte migration using time‐lapse videomicroscopy showed that SCF significantly increased migration of neonatal, but not fetal, melanocytes on fibronectin (FN). We conclude that SCF regulates integrin expression at the protein level and that SCF has pleiotropic effects on melanocyte attachment and migration on ECM ligands. We suggest that this may be one mechanism by which SCF regulates melanocyte migration during development of the skin.