Mechanism of ER stress-mediated ER-phagy by CdTe-QDs in yeast cells
Mechanism of ER stress-mediated ER-phagy by CdTe-QDs in yeast cells
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酵母细胞中 CdTe-QDs 内质网应激介导的内质网自噬机制
DOI：
10.1016/j.toxlet.2022.05.010
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发表时间：
2022
影响因子：
3.5
通讯作者：
Yixiang Duan
中科院分区：
文献类型：
--
作者：
Fujing Wei;Aimin Yang;Zhao Zhao;Huifang An;Yu Li;Yixiang Duan
内质网自噬(ER-phagy)是细胞对抗内质网应激、维持内质网内稳态的重要策略。内质网应激被认为是纳米颗粒的一种潜在毒性，但只有少数研究探讨了纳米颗粒诱导的内质网应激是否会触发内质网吞噬，而纳米颗粒诱导的内质网应激介导的内质网吞噬的确切分子机制尚不清楚。因此，我们的研究主要集中在内质网应激与内质网吞噬效应之间的关系及其分子机制上。结果表明，CdTe-量子点诱导的ROS和ER应激的积累促进了自噬和ER吞噬的激活。重要的是，我们的研究揭示了CdTe-Qds通过上调核心自噬机制的转录来激活自噬。结果表明，诱导的内质网吞噬是由Atg11/Atg40/Lst1-Sec23基因介导的，而不是由自噬机制基因介导的。我们推测，CdTe-量子点引起的ER吞噬可能包括微观ER吞噬和宏观ER吞噬。总之，这项工作为镉镉量子点在生物学领域的应用提供了有价值的信息，并为进一步了解内质网吞噬作用提供了理论基础。
Endoplasmic reticulum autophagy (ER-phagy) is an important strategy for cells against ER stress and maintain ER homeostasis. ER stress is considered as a potential toxicity of nanoparticles, but only a few studies have explored whether the nanoparticles-induced ER stress can trigger ER-phagy, and the precise molecular mechanism of ER-phagy mediated by nanoparticle-induced ER stress is still poorly understood. Therefore, our study focuses on the relationship between ER stress and ER-phagy caused by emerging nanoparticles CdTe-QDs and its molecular mechanism. The results showed that the accumulation of ROS and ER stress induced by CdTe-QDs contributed to the activation of autophagy and ER-phagy. Importantly, our study unraveled that CdTe-QDs activate autophagy by up-regulating the transcription of core autophagy machinery. It was found that the induced ER-phagy was mediated by Atg11/Atg40/Lst1-Sec23 instead of the autophagy machinery genes. We speculated that the ER-phagy caused by CdTe-QDs may include micro-ER-phagy and macro-ER-phagy. Collectively, this work provided valuable information for the application of CdTe-QDs in the field of biology and a theoretical basis for further understanding of ER-phagy.