Expression analysis of defence-related genes in grapevine leaves after inoculation with a host and a non-host pathogen
Expression analysis of defence-related genes in grapevine leaves after inoculation with a host and a non-host pathogen
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DOI：
10.1016/j.plaphy.2006.01.008
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发表时间：
2006-01-01
影响因子：
6.5
通讯作者：
Kortekamp, A
中科院分区：
文献类型：
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作者：
Kortekamp, A
分析了PR蛋白编码基因和苯丙烷代谢相关基因在感病和抗病葡萄藤叶片中的表达。响应于接种宿主病原体葡萄生霜霉病菌和非宿主病原体黄瓜霜霉病菌（Pseudoperonospora cubensis），黄瓜的霜霉病病原体。进行这些实验以阐明对霜霉病敏感的葡萄藤植物在接种非宿主病原体后是否表现出相同的防御反应。防御相关基因的表达分析表明，感病品种雷司令（葡萄属viniftra）和抗病品种蒙彼利埃荣耀（葡萄属riparia）之间的显着差异。而一些基因似乎是组成型表达的'荣耀'或诱导接种后与两种病原体，防御相关基因的表达雷司令主要是接种后的非宿主病原体的影响：PR-2，PR-3，PR-4，PGIP基因，特别是基因编码的酶参与花青素的生物合成（DFR，F3 H，LDOX）受到影响。因此，采用适当的组织学染色技术研究了接种叶片中相应产物（黄烷和其他酚类化合物）的发生情况。这些染色揭示了生产儿茶素和相关的酚类化合物的第一个48海（接种后小时）与PS。在雷司令中没有观察到葡萄生疫霉的进一步产生，而在Gloire中没有观察到进一步产生，这可能是由于在对照叶中观察到的高含量的多酚。除了染色程序之外，在叶圆片上监测孢子形成强度。用PS. cubensis导致Gloire的布朗宁反应减少（作为过敏反应的结果），并且在随后接种葡萄生疫霉后显著降低了雷司令的孢子形成强度。(c)2006年，Elsevier SAS。All rights reserved.
The expression of PR protein encoding genes and genes involved in the phenylpropanoid metabolism was analysed on grapevine leaves of susceptible and resistant cvs. in response to inoculation with the host-pathogen Plasmopara viticola and the non-host pathogen Pseudoperonospora cubensis, the downy mildew pathogen of cucumber. These experiments were conducted to elucidate whether or not grapevine plants susceptible to downy mildew exhibit an identical defence response after inoculation with the non-host pathogen. Expression analysis of defence-related genes revealed marked differences between the susceptible cultivar 'Riesling' (Vitis viniftra) and the resistant cultivar 'Gloire de Montpellier' (Vitis riparia). Whereas some genes seem to be expressed constitutively in 'Gloire' or induced after an inoculation with both pathogens, expression of defence-related genes in Riesling was influenced mainly after inoculation with the non-host pathogen: PR-2, PR-3, PR-4, a PGIP gene, and especially genes encoding enzymes involved in anthocyanin biosynthesis (DFR, F3H, LDOX) were affected. Therefore, the occurrence of the respective products (flavans and other phenolics) in inoculated leaves was investigated with appropriate histological staining techniques. These stainings revealed a production of catechins and related phenolic compounds within the first 48 hai (hours after inoculation) with Ps. cubensis but not with P viticola in Riesling, whereas in Gloire no further production was seen, which may be due to the high content of polyphenolics as observed in control leaves. In addition to the staining procedures, sporulation intensity was monitored on leaf discs. Pretreatments of leaf discs with Ps. cubensis led to a reduced browning reaction (as a result of a hypersensitive reaction) in Gloire and significantly reduced the intensity of sporulation in Riesling after a subsequent inoculation with P. viticola. (c) 2006 Elsevier SAS. All rights reserved.