A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study
A Comparison of 1-and 3.2-MHz Low-Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo Study
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DOI：
10.1002/jum.14683
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发表时间：
2019-01-01
影响因子：
2.3
通讯作者：
Wu, Lin
中科院分区：
文献类型：
--
作者：
Feng, Lifang;Liu, Xiaohan;Wu, Lin
目的低强度脉冲超声（LIPUS）结合多孔支架可作为一种新的治疗骨缺损的方法。本研究的目的是评价1和3.2 MHz LIPUS对多孔Ti64合金支架上成骨的影响，用于体外和体内研究。方法将支架材料随机分为高频超声组、低频超声组和对照组。将小鼠前成骨细胞与多孔Ti-6Al-4V支架体外培养，以评价细胞增殖和分化。此外，将支架植入兔下颌骨缺损体内。通过显微CT、甲苯胺蓝染色和von Kossa染色观察LIPUS对骨再生的影响。结果超声组与对照组细胞计数值无显著性差异（P > 0.05）。与对照组相比，超声可促进支架上细胞的碱性磷酸酶活性和骨钙素水平（P <0.05），但两种频率之间无显著性差异。此外，组织形态学分析显示，与对照组相比，超声组的新骨形成体积和数量增加，骨成熟度提高，但两种频率之间没有显着差异。结论在本实验条件下，LIPUS可促进多孔Ti64支架上成骨细胞的分化，促进骨成熟。两个频率之间没有显著差异。
Objectives Low-intensity pulsed ultrasound (LIPUS) combined with porous scaffolds can be used as a new therapy to treat bone defect repair. The aim of this study was to evaluate the effects of 1 and 3.2 MHz LIPUS on osteogenesis on porous Ti64 alloy scaffolds for both in vitro and in vivo studies. Methods Scaffolds were randomly divided into the high-frequency ultrasound group, low-frequency ultrasound group, and control group. Mouse pre-osteoblast cells were cultured with porous Ti-6Al-4V scaffolds in vitro to evaluate cell proliferation and differentiation. In addition, scaffolds were implanted into rabbit mandibular defects in vivo. The effects of LIPUS on bone regeneration were evaluated by observing the micro-computed tomography (micro-CT), toluidine blue staining, and von Kossa staining. Results The results revealed no significant difference in the cell counting kit-8 values between the ultrasound groups and control groups (P > .05). Compared with the control group, ultrasound promoted alkaline phosphatase activity and osteocalcin levels of the cells on the scaffolds (P < .05), but there was no significant difference between the two frequencies. In addition, histomorphologic analyses revealed that the volume and amount of new bone formation increased and that bone maturity improved in the ultrasound groups compared with the control group, but no significant difference was noted between the two frequencies. Conclusions Under the present experimental conditions, LIPUS promoted osteoblast differentiation and promoted bone maturity on porous Ti64 scaffolds. No significant differences were noted between the two frequencies.