Adolescence in Tetrahymena thermophila.
Adolescence in Tetrahymena thermophila.
复制标题
嗜热四膜虫的青春期。
DOI：
10.1073/pnas.82.2.436
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发表时间：
1985
影响因子：
11.1
通讯作者：
Karrer,KM
中科院分区：
文献类型：
--
作者：
Rogers,MB;Karrer,KM
嗜热四膜虫的性成熟发育包括一个中间阶段，即青春期，在此期间细胞能够与成熟细胞交配，但不能与其他青春期细胞交配。当成功交配的细胞的后代以对数生长并经常测试交配能力时，它们在大约 65 代的时间内无法形成交配对。这个时期被称为不成熟期。在下一阶段，后代与成熟细胞配对，但不与其他青春期细胞配对，尽管存在互补的交配类型。在细胞成熟之前，青春期持续 20-25 代，成熟被定义为与任何不同交配类型的细胞交配的能力。一旦与成熟细胞配对，青少年就会成功完成接合。细胞学制备表明，这对成员都经历减数分裂并形成大核原基。通过二维凝胶分析确定，青少年细胞和成熟细胞交配期间合成的蛋白质与成熟细胞交配期间合成的蛋白质相似。青春期细胞和成熟伴侣都为后代提供遗传标记。
The development of sexual maturity in Tetrahymena thermophila has been shown to include an intermediate stage, adolescence, during which cells are capable of mating with mature cells but not other adolescent cells. When the progeny of successfully mated cells are grown logarithmically and tested frequently for the ability to mate, they are unable to form mating pairs for about 65 generations. This period is known as immaturity. During the next stage, the progeny pair with mature cells but not with other adolescent cells despite the presence of complementary mating types. Adolescence persists for 20-25 generations before the cells attain maturity, which is defined as the ability to mate with any cell of different mating type. Once paired with mature cells, adolescents successfully complete conjugation. Cytological preparations show that both members of the pair undergo meiosis and form macronuclear anlagen. The proteins synthesized during a mating between adolescents and mature cells are similar to those synthesized during a mating between mature cells as determined by two-dimensional gel analysis. Both the adolescent cell and the mature partner contribute genetic markers to the progeny.