Assay of matrix metalloproteases types 8 and 9 by ELISA in human breast cancer.
Assay of matrix metalloproteases types 8 and 9 by ELISA in human breast cancer.
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DOI：
10.1038/bjc.1995.197
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发表时间：
1995-05
影响因子：
8.8
通讯作者：
Tschesche, H
中科院分区：
文献类型：
--
作者：
Duffy, M J;Blaser, J;Duggan, C;McDermott, E;O'Higgins, N;Fennelly, J J;Tschesche, H
模型肿瘤系统的结果表明，某些金属蛋白酶（MMPs）水平的增加或其抑制剂水平的降低与转移潜力相关。本研究采用酶联免疫吸附法测定了人乳腺肿瘤组织中MMP-8和-9及其抑制剂组织金属蛋白酶1型抑制剂（TIMP-1）的水平。MMP-8和-9与尿激酶纤溶酶原激活剂水平无相关性。MMP-8和-9水平也与TIMP-1水平显著相关。相反，MMP与纤溶酶原激活物抑制剂均不相关。MMP-8、MMP-9或TIMP-1与肿瘤大小或腋窝淋巴结转移没有关系。MMP-8和-9水平与雌激素受体水平呈负相关。MMP-8水平与孕酮受体水平呈负相关，而MMP-9水平与孕酮受体水平呈负相关。结论是，本文描述的MMP-8和-9的测定将允许评估这些蛋白酶作为癌症的预后标志物。
Results from model tumour systems suggest that either increased levels of certain metalloproteases (MMPs) or decreased levels of their inhibitors correlate with metastatic potential. In this study, levels of two MMPs, i.e. MMP-8 and -9, and their inhibitor tissue inhibitor of metalloprotease type 1 (TIMP-1) were measured by enzyme-linked immunosorbent assay in human breast tumours. Levels of MMP-8 and -9 correlated significantly with each other, but neither MMP correlated with urokinase plasminogen activator. Levels of both MMP-8 and -9 were also significantly related to levels of TIMP-1. In contrast, neither MMP correlated with plasminogen activator inhibitor. No relationship was found between MMP-8, MMP-9 or TIMP-1 and either tumour size or metastasis to axillary nodes. MMP-8 and -9 levels were inversely related to levels of oestrogen receptors. MMP-8 but not MMP-9 levels were also inversely correlated with progesterone receptor levels. It is concluded that the assay for MMP-8 and -9 described here will permit the evaluation of these proteases as prognostic markers in cancer.