Antiviral and Antibacterial Nanostructured Surfaces with Excellent Mechanical Properties for Hospital Applications
Antiviral and Antibacterial Nanostructured Surfaces with Excellent Mechanical Properties for Hospital Applications
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DOI：
10.1021/acsbiomaterials.0c00348
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发表时间：
2020-06-01
影响因子：
5.8
通讯作者：
Yarlagadda, Prasad K. D., V
中科院分区：
文献类型：
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作者：
Hasan, Jafar;Xu, Yanan;Yarlagadda, Prasad K. D., V
随着细菌和病毒感染的增加，包括最近爆发的冠状病毒，对新型抗微生物策略的需求也在紧迫地上升。为了解决这一问题，我们采用湿法蚀刻技术在铝（Al） 6063合金表面制备了23 nm宽的纳米结构，这些纳米结构随机排列成脊状。刻蚀时间分别为0.5、1和3 h。利用扫描电子显微镜、能量色散x射线光谱、接触角测量、纳米压痕和原子力显微镜对表面进行了表征。用革兰氏阴性菌铜绿假单胞菌和革兰氏阳性菌金黄色葡萄球菌评价细菌的附着行为。首次研究了常见的呼吸道病毒，呼吸道合胞病毒（RSV）和鼻病毒（RV）在纳米结构表面的抗病毒活性。结果表明，随着刻蚀时间的延长，铝表面具有亲水性，纳米尺度粗糙度增强，R-rms范围为69.9 ~ 995 nm。铜绿假单胞菌（P. aeruginosa）和金黄色葡萄球菌（S. aureus）的细菌细胞在蚀刻的Al 6063表面附着3 h后均发生物理变形，无法存活。这种纳米级的表面形貌分别使附着的铜绿假单胞菌和金黄色葡萄球菌细胞失活92%和87%。与光滑的人工智能对照表面相比，暴露于纳米结构表面2小时内，传染性RSV的恢复也显著减少。在纳米结构表面24小时后，鼻病毒的活力计数降低了3-4 log（10）。纳米结构的表面表现出优异的耐久性，可以承受2000亩氮载荷1000次而没有任何损伤。这是第一份显示纳米结构表面具有抗菌和抗病毒特性的报告，该纳米结构表面具有优异的纳米力学特性，在医院环境中用于阻止物理表面引起的感染传播可能具有潜在的重要意义。
With the rise of bacterial and viral infections including the recent outbreak of coronavirus, the requirement for novel antimicrobial strategies is also rising with urgency. To solve this problem, we have used a wet etching technique to fabricate 23 nm wide nanostructures randomly aligned as ridges on aluminum (Al) 6063 alloy surfaces. The surfaces were etched for 0.5, 1, and 3 h. The surfaces were characterized using scanning electron microscopy, energy-dispersive X-ray spectroscopy, contact angle goniometry, nanoindentation and atomic force microscopy. Strains of the Gram negative bacteria Pseudomonas aeruginosa and the Gram positive bacteria Staphylococcus aureus were used to evaluate the bacterial attachment behavior. For the first time, common respiratory viruses, respiratory syncytial virus (RSV) and rhinovirus (RV), were investigated for antiviral activity on nanostructured surfaces. It was found that the etched Al surfaces were hydrophilic and the nanoscale roughness enhanced with the etching time with R-rms ranging from 69.9 to 995 nm. Both bacterial cells of P. aeruginosa and S. aureus were physically deformed and were nonviable upon attachment after 3 h on the etched Al 6063 surface. This nanoscale surface topography inactivated 92 and 87% of the attached P. aeruginosa and S. aureus cells, respectively. The recovery of infectious RSV was also reduced significantly within 2 h of exposure to the nanostructured surfaces compared to the smooth Al control surfaces. There was a 3-4 log(10) reduction in the viability counts of rhinovirus after 24 h on the nanostructured surfaces. The nanostructured surfaces exhibited excellent durability as the surfaces sustained 1000 cycles of 2000 mu N load without any damage. This is the first report that has shown the combined antibacterial and antiviral property of the nanostructured surface with excellent nanomechanical properties that could be potentially significant for use in hospital environments to stop the spread of infections arising from physical surfaces.