Genetic Characteristics of Conditional Lethal Mutants of Vesicular Stomatitis Virus Induced by 5-Fluorouracil, 5-Azacytidine, and Ethyl Methane Sulfonate
Genetic Characteristics of Conditional Lethal Mutants of Vesicular Stomatitis Virus Induced by 5-Fluorouracil, 5-Azacytidine, and Ethyl Methane Sulfonate
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5-氟尿嘧啶、5-氮杂胞苷和甲磺酸乙酯诱导的水疱性口炎病毒条件致死突变体的遗传特征
DOI：
10.1128/jvi.5.5.559-567.1970
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发表时间：
1970
影响因子：
5.4
通讯作者：
C. Pringle
中科院分区：
文献类型：
--
作者：
C. Pringle
用5-氟尿嘧啶（FU）、5-氮胞苷（ACR）和甲基磺酸乙酯（EMS）诱发的175株水泡性口炎病毒（Indiana-C型）温度敏感（TS）突变株，通过定性试验分为4个互补组。第一组包含151个突变体;第二组，2个突变体;第三组，1个突变体;第四组，15个突变体; 6个未分类。FU作为诱变剂比ACR或EMS更有效。然而，在所有三种诱变剂的情况下，属于组I和组IV的突变体的比例相似。来自组I和组IV的15个突变体已被用于获得定量互补数据。这两个群体似乎是同质的。互补产量随着多样性的增加而增加，但引起最大互补所需的每个细胞的颗粒数很小。基因重组的模式与互补的模式相似。在组I（<0.001%）或组IV（<0.07%）内的杂交中未检测到重组，而在组I和组IV之间的杂交中观察到重组（0.31至3.4%）。在每细胞输入0.6个噬菌斑形成单位以上时，重复频率并没有增加。许多组I突变体具有非常低的回复率，并且被这些突变体之一感染的BHK 21克隆13细胞通过在限制性温度下长时间暴露而被“治愈”感染。
One hundred and seventy-five temperature-sensitive (ts) mutants of vesicular stomatitis virus (type Indiana-C) induced by 5-fluorouracil (FU), 5-azacytidine (ACR), and ethyl methane sulfonate (EMS) have been assigned to four complementation groups by a qualitative test. Group I contains 151 mutants; group II, 2 mutants; group III, 1 mutant; and group IV, 15 mutants; 6 are unclassified. FU was much more effective as a mutagen than either ACR or EMS. The proportion of the mutants belonging to groups I and IV, however, was similar in the case of all three mutagens. Fifteen mutants from groups I and IV have been used to obtain quantitative complementation data. Both groups appear to be homogeneous. Complementation yields increase with increasing multiplicity, but the number of particles per cell required to elicit maximal complementation is small. The pattern of genetic recombination parallels that of complementation. No recombination could be detected in crosses within group I (<0.001%) or group IV (<0.07%), whereas recombination (0.31 to 3.4%) was observed in crosses between groups I and IV. Recombination frequency did not increase with multiplicity above an input of 0.6 plaque-forming units per cell. Many group I mutants have very low reversion rates, and BHK 21 clone 13 cells infected with one of these mutants have been “cured” of infection by prolonged exposure at the restrictive temperature.