Complex structure and regulation of the ABP/SHBG gene.
Complex structure and regulation of the ABP/SHBG gene.
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ABP/SHBG 基因的复杂结构和调控。
DOI：
10.1016/0960-0760(91)90302-l
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Bayliss,DM
中科院分区：
文献类型：
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作者：
Joseph,DR;Sullivan,PM;Wang,YM;Millhorn,DE;Bayliss,DM
细胞外雄激素结合蛋白（ABPs）被认为调节雄激素和反式作用的核雄激素受体的调节功能。睾丸ABP和血浆性激素结合球蛋白（SHBG），这是在肝脏中产生的，是由相同的基因编码。我们在这里报告，ABP/SHBG基因也表达在胎鼠肝脏和成年大脑。免疫反应性ABP位于脑和胎肝中，通过北方印迹杂交在两种组织中鉴定了mRNA。脑和胎肝cDNA克隆的分析揭示了具有序列特征的交替加工RNA，这表明编码的蛋白质可以作为ABP/SHBG与细胞表面受体结合的竞争者。一个cDNA代表ABP/SHBG基因和组氨酸脱羧酶基因的融合转录本，该基因显然是通过反式剪接过程形成的。基因测序实验表明，组织特异性ABP/SHBG基因启动子-增强子元件在睾丸、脑和胎肝中被利用。这些数据表明ABP/SHBG基因的结构、RNA转录加工和可能的调控非常复杂。
Extracellular androgen-binding proteins (ABPs) are thought to modulate the regulatory functions of androgens and the trans-acting nuclear androgen receptor. Testicular ABP and plasma sex hormone-binding globulin (SHBG), which is produced in the liver, are encoded by the same gene. We report here that the ABP/SHBG gene is also expressed in fetal rat liver and adult brain. Immunoreactive ABP was localized in the brain and fetal liver and mRNAs were identified in both tissues by northern blot hybridization. Analysis of brain and fetal liver cDNA clones revealed alternatively processed RNAs with sequence characteristics suggesting the encoded proteins could act as competitors of ABP/SHBG binding to cell surface receptors. One cDNA represented a fused transcript of the ABP/SHBG gene and the histidine decarboxylase gene that was apparently formed by a trans-splicing process. Gene sequencing experiments indicate that tissue-specific ABP/SHBG gene promoter-enhancer elements are utilized in testis, brain and fetal liver. These data demonstrate that the structure, RNA transcript processing and likely regulation of the ABP/SHBG gene are very complex.