Detection of T lymphocyte subsets and related functional molecules in follicular fluid of patients with polycystic ovary syndrome
Detection of T lymphocyte subsets and related functional molecules in follicular fluid of patients with polycystic ovary syndrome
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多囊卵巢综合征患者卵泡液中T淋巴细胞亚群及相关功能分子的检测
DOI：
10.1038/s41598-019-42631-x
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发表时间：
2019-04-15
影响因子：
4.6
通讯作者：
Yang, Quan
中科院分区：
文献类型：
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作者：
Li, Zitao;Peng, Anping;Yang, Quan
免疫应答在多囊卵巢综合征（PCOS）的发病机制中起重要作用。然而，对PCOS患者T淋巴细胞亚群的特点仍缺乏足够的了解。本研究采用体外受精（IVF）前取卵的卵泡液（FF）中的淋巴细胞，对PCOS患者和非PCOS患者进行比较。流式细胞术检测T淋巴细胞分化抗原25（CD 25）、CD 69、程序性死亡蛋白1（PD-1）、γ干扰素（IFN-γ）、白细胞介素17 A（IL-17 A）和IL-10的表达水平。结果表明，PCOS不孕患者外周血FF CD 8 +T细胞比例明显降低（P< 0.05）。PCOS不孕患者外周血CD 4+、CD 8 +T细胞中CD 69、IFN-γ水平均显著降低，PD-1水平显著升高（P< 0.05）。PCOS不孕患者外周血CD 4+、CD 8 +T细胞PD-1表达与血清雌二醇（E2）水平呈正相关，与CD 4+、CD 8 +T细胞IFN-γ表达呈正相关。提示T细胞功能障碍可能参与了PCOS的发病过程。
Immune responses play an important role in the pathogenesis of polycystic ovary syndrome (PCOS). However, the characteristics of T lymphocyte subsets in PCOS remain insufficiently understood. In this study, lymphocytes of follicular fluid (FF) were obtained from oocyte retrieval beforein-vitrofertilization (IVF) in infertile women with or without PCOS. The levels of cluster of differentiation 25 (CD25), CD69, programmed death 1 (PD-1), interferon-γ (IFN-γ), interleukin 17A (IL-17A) and IL-10 in T lymphocytes were determined by flow cytometry. Our results showed that the percentage of FF CD8+T cells was significantly decreased in infertile patients with PCOS (P< 0.05). Furthermore, the levels of CD69 and IFN-γ were significantly decreased and the level of PD-1 was increased in both CD4+and CD8+T cells from infertile patients with PCOS (P< 0.05). Moreover, the expression of PD-1 on CD4+or CD8+T cells was positively correlated with the estradiol (E2) levels in the serum and reversely correlated with the expression of IFN-γ in CD4+or CD8+T cells in infertile patients with PCOS. These results suggested that T cell dysfunction may be involved in the pathogenesis of PCOS.