Dynein anchors its mRNA cargo after apical transport in the Drosophila blastoderm embryo
Dynein anchors its mRNA cargo after apical transport in the Drosophila blastoderm embryo
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DOI：
10.1016/j.cell.2005.04.033
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发表时间：
2005-07-15
期刊：
影响因子：
64.5
通讯作者：
Davis, I
中科院分区：
文献类型：
--
作者：
Delanoue, R;Davis, I
分子马达在许多生物体中沿着细胞骨架积极地运输许多类型的货物沿着。一类货物是局部mRNA，其通过肌动蛋白丝上的肌球蛋白或微管上的驱动蛋白和动力蛋白转运。货物是如何保持在其最终的细胞内目的地，以及在运输完成后马达是否再循环，目前还知之甚少。在这里，我们使用一种新的RNA锚定在活的果蝇胚盘胚胎显示顶端锚定的mRNA后，动力蛋白运输完成不依赖于肌动蛋白或连续主动运输的电机。相反，RNA的顶端锚定需要微管，并涉及动力蛋白作为静态锚，与货物保持在其最终目的地。我们提出了一个普遍的原则，也可以适用于其他动力蛋白货物和其他一些分子马达，货物运输和锚定驻留在同一个分子。
Molecular motors actively transport many types of cargo along the cytoskeleton in a wide range of organisms. One class of cargo is localized mRNAs, which are transported by myosin on actin filaments or by kinesin and dynein on microtubules. How the cargo is kept at its final intracellular destination and whether the motors are recycled after completion of transport are poorly understood. Here, we use a new RNA anchoring assay in living Drosophila blastoderm embryos to show that apical anchoring of mRNA after completion of dynein transport does not depend on actin or on continuous active transport by the motor. Instead, apical anchoring of RNA requires microtubules and involves dynein as a static anchor that remains with the cargo at its final destination. We propose a general principle that could also apply to other dynein cargo and to some other molecular motors, whereby cargo transport and anchoring reside in the same molecule.