Isolation and characterization of microsatellite DNA loci in Japanese flounder Paralichthys olivaceus (Pleuronectiformes, Pleuronectoidei, Paralichthyidae)
Isolation and characterization of microsatellite DNA loci in Japanese flounder Paralichthys olivaceus (Pleuronectiformes, Pleuronectoidei, Paralichthyidae)
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DOI：
10.1046/j.1365-294x.2000.105325.x
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发表时间：
2000-12-01
影响因子：
4.9
通讯作者：
Hara, M
中科院分区：
文献类型：
--
作者：
Sekino, M;Hara, M
构建了富含（CA）n的牙鲆基因组文库。对来自该文库的80个克隆进行测序; 59个克隆含有重复序列。设计PCR引物扩增其中27个位点。对64-69尾鱼的16个基因座（Po 1、Po 13、Po 20、Po 25 A、Po 26、Po 31、Po 33、Po 35、Po 42、Po 48、Po 52、Po 56、Po 58、Po 83、Po 89和Po 91）的多态性进行了详细分析。等位基因数为4 ~ 40个，观察杂合度为0.43 ~ 0.99，期望杂合度为0.43 ~ 0.97。只有Po 31偏离Hardy-Weinberg平衡。所报道的核苷酸序列已保藏于DDBJ/EMBL/GenBank数据库，登录号为AB 046745-AB 046746和AB 046748-AB 046761。
A (CA)n-enriched Japanese flounder genomic library was produced. 80 clones from this library were sequenced; 59 clones contained repeat sequences. PCR primers were designed to amplify 27 of these loci. Polymorphisms of 16 loci (Po1, Po13, Po20, Po25A, Po26, Po31, Po33, Po35, Po42, Po48, Po52, Po56, Po58, Po83, Po89andPo91) in 64-69 fish were analysed in detail. The number of alleles ranged from 4 to 40, the observed heterozygosity ranged from 0.43 to 0.99 and the expected heterozygosity ranged from 0.43 to 0.97. OnlyPo31displayed significant deviations from Hardy-Weinberg equilibrium. The nucleotide sequences reported have been deposited with the DDBJ/EMBL/GenBank databases with the accession numbers AB046745-AB046746 and AB046748-AB046761.