Sublocalization of an invasion-inducing locus and other genes on human chromosome 7.
Sublocalization of an invasion-inducing locus and other genes on human chromosome 7.
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人类 7 号染色体上的入侵诱导位点和其他基因的亚定位。
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
John G. Collard
中科院分区：
文献类型：
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作者：
G. Habets;R. A. van der Kammen;V. Willemsen;M. Balemans;J. Wiegant;John G. Collard
通过非侵袭性小鼠 T 淋巴瘤细胞和侵袭性人类激活的正常 T 细胞之间的体细胞融合研究，我们之前已经表明，负责在种间 T 细胞杂交中诱导侵袭和转移潜力的遗传信息位于人类 7 号染色体上。显然，源自正常活化 T 细胞的基因在杂交中主要表达，并控制这些 T 淋巴瘤细胞的侵袭和转移潜力。为了对 7 号染色体上的入侵诱导位点进行亚定位，我们生成了仅包含人类 7 号染色体特定区域的杂交体，带有或不带有人类 21 号染色体的小片段。对这些杂交体的分析表明，入侵诱导位点映射到 7p12----cen。使用大量 7 号染色体特异性 DNA 探针通过 Southern 印迹分析证实了杂交体的人类 DNA 互补体。其中一些基因可以进一步亚定位。这些包括：ARAF2至7p12----cen、D7S21至7pter----p12、ACTB至7p15----p12、EGFR至7p12、MDH2至7cen----q22和PDGFA至7pter----p15。
By somatic cell fusion studies between noninvasive mouse T-lymphoma cells and invasive human activated normal T-cells we have previously shown that the genetic information responsible for the induction of invasive and metastatic potential in interspecies T-cell hybrids is located on human chromosome 7. Apparently, genes derived from normal activated T-cells are dominantly expressed in the hybrids and control the invasive and, as a consequence, metastatic potential of these T-lymphoma cells. To sublocalize the invasion-inducing locus on chromosome 7 we have generated hybrids that harbor only specific regions of human chromosome 7 with or without a small fragment of human chromosome 21. Analysis of these hybrids revealed that the invasion-inducing locus maps to 7p12----cen. The human DNA complement of the hybrids was confirmed by Southern blot analysis using a large panel of chromosome 7-specific DNA probes. Several of these genes could be further sublocalized. These included: ARAF2 to 7p12----cen, D7S21 to 7pter----p12, ACTB to 7p15----p12, EGFR to 7p12, MDH2 to 7cen----q22, and PDGFA to 7pter----p15.