EFFECTS OF ISOLEUCINE DEFICIENCY ON NUCLEIC-ACID AND PROTEIN-METABOLISM IN CULTURED CHINESE-HAMSTER CELLS - CONTINUED RIBONUCLEIC-ACID AND PROTEIN-SYNTHESIS IN ABSENCE OF DEOXYRIBONUCLEIC ACID SYNTHESIS
EFFECTS OF ISOLEUCINE DEFICIENCY ON NUCLEIC-ACID AND PROTEIN-METABOLISM IN CULTURED CHINESE-HAMSTER CELLS - CONTINUED RIBONUCLEIC-ACID AND PROTEIN-SYNTHESIS IN ABSENCE OF DEOXYRIBONUCLEIC ACID SYNTHESIS
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DOI：
10.1021/bi00752a019
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
TOBEY, RA
中科院分区：
文献类型：
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作者：
ENGER, MD;TOBEY, RA
材料和方法细胞和培养基。如前所述（Tobey和Ley，1971），在F-10培养基中悬浮培养CHO细胞的亚二倍体（众数染色体数为21）系，使其不含PPLO。为了将细胞转移到异亮氨酸缺乏培养基中，从储备悬浮液中取出等分试样。
Materials and MethodsCells and Media. A hypodiploid (modal chromosome num-ber of 21) line of CHO cells was maintained free of PPLO in suspension culturein F-10 medium, as described previously (Tobey and Ley, 1971). For transfer of cells to isoleucinedeficient medium, aliquots were removed from stock suspen-