REGULATION OF GALACTOSE OPERON EXPRESSION - GLUCOSE EFFECTS AND ROLE OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE
REGULATION OF GALACTOSE OPERON EXPRESSION - GLUCOSE EFFECTS AND ROLE OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE
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DOI：
10.1128/jb.146.1.149-154.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
ULLMANN, A
中科院分区：
文献类型：
--
作者：
JOSEPH, E;DANCHIN, A;ULLMANN, A
研究了葡萄糖在大肠杆菌K-12中对半乳糖操纵子表达的影响：分解代谢物抑制和诱导剂排斥。使用诱导菌株和组成菌株并测量启动子-近端酶合成速率，半乳糖操纵子似乎没有表现出分解代谢抑制。葡萄糖对半乳糖操纵子表达的唯一影响是诱导剂的排除，这表明在葡萄糖和半乳糖存在的情况下存在双氧生长。这种双重作用并没有被cAMP缓解，cAMP似乎并不是葡萄糖对半乳糖操纵子表达的拮抗剂。cAMP的唯一作用是刺激启动子远端基因的表达。
The following 2 aspects of the glucose effect on galactose operon expression were studied in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, the galactose operon did not seem to exhibit catabolite repression. The only glucose effect on galactose operon expression observed was inducer exclusion, as shown by the existence of diauxic growth in the presence of glucose and galactose. This diauxie was not relieved by cAMP which did not seem to be an antagonist of any glucose effect on galactose operon expression. The only effect of cAMP was to stimulate promoter-distal gene expression.