Comparative Effects of Osmotic, Sodium Nitrite-Induced, and pH-Induced Stress on Growth and Survival of Clostridium perfringens Type A Isolates Carrying Chromosomal or Plasmid-Borne Enterotoxin Genes
Comparative Effects of Osmotic, Sodium Nitrite-Induced, and pH-Induced Stress on Growth and Survival of Clostridium perfringens Type A Isolates Carrying Chromosomal or Plasmid-Borne Enterotoxin Genes
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渗透压、亚硝酸钠诱导和 pH 诱导应激对携带染色体或质粒携带肠毒素基因的 A 型产气荚膜梭菌分离株生长和存活的比较影响
DOI：
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发表时间：
2006
影响因子：
4.4
通讯作者：
B. McClane
中科院分区：
文献类型：
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作者：
Jihong Li;B. McClane
摘要产气荚膜梭菌中约有1%~ 2%的分离株携带引起C. A型产气荚膜杆菌食物中毒虽然cpe基因可以是染色体或质粒携带的，但食物中毒分离株通常携带染色体cpe基因。先前的研究已经将染色体CPE分离株之间的这种关联联系起来（即，C-cpe分离物）和食物中毒，至少部分地归因于C-cpe分离物的孢子和营养细胞特别耐高温和低温。目前的研究表明，C-cpe分离株的抗性表型不仅包括温度抗性，还包括营养细胞和孢子对渗透胁迫（来自NaCl）和亚硝酸盐的抗性增强。然而，通过省略一个离群分离株，没有显着差异的pH敏感性检测孢子或营养细胞的C-cpe分离株与携带质粒携带cpe基因的分离株。这些结果表明，C-cpe分离物的营养细胞和孢子对几种食品保存方法以及极端温度都具有不寻常的抗性。C-cpe耐药表型的广谱性质表明，这些细菌可能采用多种机制在传播给人类之前在食物中持续存在和生长。
ABSTRACT About 1 to 2% of Clostridium perfringens isolates carry the enterotoxin gene (cpe) necessary for causing C. perfringens type A food poisoning. While the cpe gene can be either chromosomal or plasmid borne, food poisoning isolates usually carry a chromosomal cpe gene. Previous studies have linked this association between chromosomal cpe isolates (i.e., C-cpe isolates) and food poisoning, at least in part, to both the spores and vegetative cells of C-cpe isolates being particularly resistant to high and low temperatures. The current study now reveals that the resistance phenotype of C-cpe isolates extends beyond temperature resistance to also include, for both vegetative cells and spores, enhanced resistance to osmotic stress (from NaCl) and nitrites. However, by omitting one outlier isolate, no significant differences in pH sensitivity were detected between the spores or vegetative cells of C-cpe isolates versus isolates carrying a plasmid-borne cpe gene. These results indicate that both vegetative cells and spores of C-cpe isolates are unusually resistant to several food preservation approaches in addition to temperature extremes. The broad-spectrum nature of the C-cpe resistance phenotype suggests these bacteria may employ multiple mechanisms to persist and grow in foods prior to their transmission to humans.
产气荚膜梭菌肠毒素和肠道紧密连接。
影响因子：
15.9
作者：
McClane,BA
通讯作者：
McClane,BA