TIMES OF GENERATION OF GLUTAMIC-ACID DECARBOXYLASE IMMUNOREACTIVE NEURONS IN MOUSE SOMATOSENSORY CORTEX
TIMES OF GENERATION OF GLUTAMIC-ACID DECARBOXYLASE IMMUNOREACTIVE NEURONS IN MOUSE SOMATOSENSORY CORTEX
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DOI：
10.1002/cne.902510105
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发表时间：
1986-09-01
影响因子：
2.5
通讯作者：
FONSECA, M
中科院分区：
文献类型：
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作者：
FAIREN, A;COBAS, A;FONSECA, M
本文报道了小鼠体感皮层谷氨酸脱羧酶（GAD）阳性神经元的出生日期。妊娠C57 B1小鼠从E10至E17（E0为交配日）接受（3 H）胸苷脉冲注射。胸苷标记的，GAD阳性和nonimmunoreactive（非GAD）细胞作为软膜表面下的深度的函数的分布记录在成年动物。GAD阳性神经元的最大生成速率发生在EW 14，而非GAD神经元的生成在E13达到其最大速率。除I层外，GAD阳性神经元的定位顺序为由内向外。在E12和E13出生的GAD阳性神经元位于VI-IV层。在E14出生的GAD阳性神经元被发现在整个皮质厚度，最大的IV层。E15、E16、E17脉冲后标记的GAD阳性神经元局限于浅层，随着脉冲标记年龄的增加，GAD阳性神经元向软膜表面移动时形成一条逐渐变窄的带。在分析的整个胚胎期，I层中的GAD阳性神经元以恒定的速率产生。非GAD神经元也遵循由内而外的时空梯度。两个部分重叠的阶段，区分在非GAD神经元。在第一阶段（从E12到E14）的神经元填充成人层VI和V起源，而位于层IV到I的神经元在第二阶段（从E13到E17）产生。由于GAD免疫反应神经元来自异质性人群，我们设想进一步的研究，以测试是否存在差异，在类之间的出生日期。
The birth dates of neurons showing glutamic acid decarboxylase (GAD) immunoreactivity have been determined in mouse somatosensory cortex. Pregnant C57B1 mice received pulse injections of (3H)thymidine from E10 through E17 (E0 being the day of mating). The distributions of thymidine-labeled, GAD-positive and nonimmunoreactive (non-GAD) cells as a function of depth under the pial surface were recorded in adult animals. The maximum rate of generation of GAD-positive neurons occurred at EW14, whereas the generation of non-GAD neurons reached its maximum rate at E13. Except for those in layer I, GAD-positive neurons followed an inside-out sequence of positioning. GAD-positive neurons born at E12 and E13 were located in layers VI-IV. GAD-positive neurons born at E14 were found throughout the cortical thickness, with a maximum in layer IV. The GAD-positive neurons labeled after pulse at E15 or E16 or E17 were limited to the superficial strata, forming a band that became narrower as it moved toward the pial surface with increase in age of pulse labeling. GAD-positive neurons in layer I were generated at a constant rate during the whole embryonic period analyzed. Non-GAD neurons also followed an inside-out spatiotemporal gradient. Two partially overlapping phases were distinguished in non-GAD neurones. During the first phase (from E12 to E14) neurons populating adult layers VI and V originated, while neurons located in layers IV through I were generated during the second phase (from E13 to E17). Since GAD-immunoreactive neurons from a heterogeneous population, we envisage further studies in order to test whether differences exist in birth dates among the classes.