Relationship between morphological taxonomy and molecular divergence within Crustacea:: Proposal of a molecular threshold to help species delimitation
Relationship between morphological taxonomy and molecular divergence within Crustacea:: Proposal of a molecular threshold to help species delimitation
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DOI：
10.1016/j.ympev.2006.03.014
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发表时间：
2006-08-01
影响因子：
4.1
通讯作者：
Gibert, J.
中科院分区：
文献类型：
--
作者：
Lefebure, T.;Douady, C. J.;Gibert, J.
随着今天的技术生产的分子序列，DNA分类和条码出现作为一个新的工具，进化生物学和生态学。然而，它们的有效性仍然需要实证评估。最重要的是形态分类学和分子分歧之间的相关性的强度和定义一些分子阈值的可能性。在这里，我们报告了甲壳动物亚门内两个线粒体基因（COI和16 S rRNA）的这种相关性的测量结果。Perl脚本的开发是为了确保我们测试的客观性、可重复性和详尽性。我们的分析揭示了分子分歧和分类之间的一般相关性。这种相关性是特别高的浅分类水平，使我们能够提出一个COI通用甲壳类阈值，以帮助物种划界。在更高的分类水平上，这种相关性降低，特别是在比较不同的科时。这些结果为DNA在分类学中的应用辩护，并提出了一种与系统发育物种定义相关的操作方法，以帮助甲壳类物种划界。这一务实的工具预计将对目前的分类进行微调，而不是像有些人认为的那样，将其撕裂。(c)2006年爱思唯尔公司All rights reserved.
With todays technology for production of molecular sequences, DNA taxonomy and barcoding arose as a new tool for evolutionary biology and ecology. However, their validities still need to be empirically evaluated. Of most importance is the strength of the correlation between morphological taxonomy and molecular divergence and the possibility to define some molecular thresholds. Here, we report measurements of this correlation for two mitochondrial genes (COI and 16S rRNA) within the sub-phylum Crustacea. Perl scripts were developed to ensure objectivity, reproducibility, and exhaustiveness of our tests. Our analysis reveals a general correlation between molecular divergence and taxonomy. This correlation is particularly high for shallow taxonomic levels allowing us to propose a COI universal crustacean threshold to help species delimitation. At higher taxonomic levels this correlation decreases, particularly when comparing different families. Those results plead for DNA use in taxonomy and suggest an operational method to help crustacean species delimitation that is linked to the phylogenetic species definition. This pragmatic tool is expected to fine tune the present classification, and not, as some would have believed, to tear it apart. (c) 2006 Elsevier Inc. All rights reserved.