DURATION OF SPERMATOGENESIS IN THE MOUSE AND TIMING OF STAGES OF THE CYCLE OF THE SEMINIFEROUS EPITHELIUM
DURATION OF SPERMATOGENESIS IN THE MOUSE AND TIMING OF STAGES OF THE CYCLE OF THE SEMINIFEROUS EPITHELIUM
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DOI：
10.1002/aja.1000990307
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发表时间：
1956-01-01
影响因子：
--
通讯作者：
OAKBERG, EF
中科院分区：
文献类型：
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作者：
OAKBERG, EF
精原细胞晚期的高辐射敏感性表明，先前对精子发生持续时间的估计仅代表A型精原细胞发育成精子所需时间的四分之三。雄性 F1 杂交小鼠 (101 x C3H) 在 12 周龄时将部分身体暴露于 100 r X 射线。暴露后 9 小时至 38 天，动物被处死。杀死中间精原细胞和 B 型精原细胞会导致 VII 期小管中静止的初级精母细胞在 48 小时内消失，在一个生精周期后粗线期丧失，在完成两个周期后丧失精细胞，在完成三个周期后丧失精子。将曲线拟合到每种细胞类型的消失率，并确定连续 50% 点之间的时间间隔。使用相同的程序来估计精细胞和精子在 25-38 天时再次出现之间的间隔。周期长度的汇总估计为 207±6 小时。由于生精上皮在 A 型精原细胞发育成精子所需的时间间隔内发生 4 个周期，因此精子发生持续时间估计为 34 1/2 天。基于 3000 个随机选择的对照小管的频率分布用于为生精上皮周期中的每个阶段分配时间间隔。
High radiation sensitivity of late spermatogonial stages indicates that previous estimates of the duration of spermatogenesis represent only three-fourths of the time required for type A spermatogonia to develop into spermatozoa. Male F1 hybrid mice (101 x C3H) were given a partial body exposure to 100 r of x rays when 12 weeks old. Animals were killed 9 hours to 38 days after exposure. Killing of intermediate and type B spermatogonia results in disappearance of resting primary spermatocytes from stage VII tubules by 48 hours, loss of pachytene stages one spermatogenic cycle later, loss of spermatids with completion of two cycles, and loss of spermatozoa after completion of three cycles. Curves were fitted to the rate of disappearance for each cell type, and time intervals between successive 50% points determined. The same procedure was used to estimate the interval between reappearance of spermatids and spermatozoa at 25-38 days. The pooled estimate of cycle length was 207 [plus or minus] 6 hours. As 4 cycles of the seminiferous epithelium occur in the interval required for type A spermatogonia to develop into sperm, the estimate of duration of spermatogenesis is 34 1/2 days. A frequency distribution based on 3000 randomly selected control tubules was used for assigning a time interval to each stage in the cycle of the seminiferous epithelium.