Comparative Evaluation of Microbiota Engraftment Following Fecal Microbiota Transfer in Mice Models: Age, Kinetic and Microbial Status Matter
Comparative Evaluation of Microbiota Engraftment Following Fecal Microbiota Transfer in Mice Models: Age, Kinetic and Microbial Status Matter
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DOI：
10.3389/fmicb.2018.03289
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发表时间：
2019-01-14
影响因子：
5.2
通讯作者：
Clement, Karine
中科院分区：
文献类型：
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作者：
Le Roy, Tiphaine;Debedat, Jean;Clement, Karine
肠道微生物群及其功能与宿主生理学错综复杂地交织在一起。用供体微生物群定殖啮齿动物提供了对宿主-微生物群相互作用表征和对疾病生理病理学的理解的见解。然而，需要对接种方法和受体小鼠模型进行更好的评估。在这里，我们比较了无菌（GF）小鼠和无特定病原体（SPF）小鼠中遗传性肥胖小鼠微生物群的短期和长期植入。我们还测试了在微生物群转移之前初始微生物群消耗的影响。在目前的工作中，供体微生物群植入在幼年SPF小鼠中比在成年SPF小鼠中更好。在幼年小鼠中，使用泻药或抗生素的初始微生物群消耗改善了微生物群转移后9周的供体微生物群植入，但不是3周。在微生物群转移后3周，微生物群耗尽的幼年小鼠表现优于GF小鼠。然而，转移后9周，定殖的GF小鼠微生物群与供体微生物群的Unifrac距离最低。定殖GF小鼠的特征还在于肠吸收功能的慢性改变。通过这些集体结果，我们表明，在微生物群转移研究中，使用经历初始微生物群耗竭的幼年小鼠构成了GF小鼠的有效替代方案。
The intestinal microbiota and its functions are intricately interwoven with host physiology. Colonizing rodents with donor microbiota provides insights into host-microbiota interactions characterization and the understanding of disease physiopathology. However, a better assessment of inoculation methods and recipient mouse models is needed. Here, we compare the engraftment at short and long term of genetically obese mice microbiota in germ-free (GF) mice and juvenile and adult specific pathogen free (SPF) mice. We also tested the effects of initial microbiota depletion before microbiota transfer. In the present work, donor microbiota engraftment was better in juvenile SPF mice than in adult SPF mice. In juvenile mice, initial microbiota depletion using laxatives or antibiotics improved donor microbiota engraftment 9 weeks but not 3 weeks after microbiota transfer. Microbiota-depleted juvenile mice performed better than GF mice 3 weeks after the microbiota transfer. However, 9 weeks after transfer, colonized GF mice microbiota had the lowest Unifrac distance to the donor microbiota. Colonized GF mice were also characterized by a chronic alteration in intestinal absorptive function. With these collective results, we show that the use of juvenile mice subjected to initial microbiota depletion constitutes a valid alternative to GF mice in microbiota transfer studies.