Cortisol promotes differentiation of epidermal ionocytes through Foxi3 transcription factors in zebrafish (Danio rerio)
Cortisol promotes differentiation of epidermal ionocytes through Foxi3 transcription factors in zebrafish (Danio rerio)
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DOI：
10.1016/j.cbpa.2012.09.011
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发表时间：
2013-01-01
影响因子：
2.3
通讯作者：
Hwang, Pung-Pung
中科院分区：
文献类型：
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作者：
Cruz, Shelly Abad;Chao, Pei-Lin;Hwang, Pung-Pung
糖皮质激素调节表皮细胞增殖，并用于治疗某些皮肤疾病。皮质醇，一种糖皮质激素，也与硬骨鱼的皮肤发育有关。在环境适应过程中，皮质醇增加了泛盐鱼类上皮离子细胞的数量，但尚不清楚这是由于分化增加还是增殖增加。为了研究，我们用外源性皮质醇（20mg /L）处理斑马鱼胚胎。皮质醇显著增加了Na+-K+-ATPase富细胞（NaRCs）和H+-ATPase富细胞（HRCs）的离子细胞密度，并伴有相应标记基因的增加。糖皮质激素受体（GR）基因表达降低。皮质醇处理还增加了培养的成年斑马鱼鳃中的离子细胞，并上调了编码叉头盒13 （foxi3a和foxi3b）转录因子的基因表达，这些转录因子调节离子细胞祖细胞的发育。体外皮质醇上调GR表达；因此，体内观察到的减少反映了调节系统的负反馈。值得注意的是，原位杂交显示，在受精后24-48 h，皮质醇增加了foxi3a/b mRNA的表达。皮质醇也减少角质形成细胞，但不影响表皮干细胞或粘液细胞。我们得出结论，皮质醇- gr激活fox3a /b有利于离子细胞祖细胞的分化，从而促进成熟离子细胞的增殖。(C) 2012爱思唯尔公司版权所有。
Glucocorticoid regulates epidermal cell proliferation, and is used to treat certain skin disorders. Cortisol, a glucocorticoid, is also linked to skin development in teleost fish. Cortisol increases the number of epithelial ionocytes during environmental acclimation in euryhaline fishes, but it is unclear whether this is due to increased differentiation or proliferation. To investigate, we treated zebrafish embryos with exogenous cortisol (20 mg/L). The densities of the ionocytes Na+-K+-ATPase rich cells (NaRCs) and H+-ATPase rich cells (HRCs) were significantly increased by cortisol, and this was accompanied by an increase in the respective marker genes. Expression of the glucocorticoid receptor (GR) gene was decreased. Cortisol treatment also increased ionocytes in cultured adult zebrafish gills, and up-regulated expression of genes encoding forkhead box 13 (foxi3a and foxi3b) transcription factors, which regulate ionocyte progenitor development. GR expression was up-regulated by cortisol in vitro; as such, the observed decrease in vivo reflects a regulatory systemic-negative feedback. Notably, in situ hybridization revealed that foxi3a/b mRNA expression was increased by cortisol at 24-48 h post-fertilization. Cortisol also decreased keratinocytes, but did not affect epidermal stem cells or mucus cells. We conclude that foxi3a/b transactivation by cortisol-GR favors differentiation of ionocyte progenitors, thereby facilitating proliferation of mature ionocytes. (C) 2012 Elsevier Inc. All rights reserved.