Fitness cost, cross resistance and realized heritability of resistance to imidacloprid in Spodoptera litura (Lepidoptera: Noctuidae)
Fitness cost, cross resistance and realized heritability of resistance to imidacloprid in Spodoptera litura (Lepidoptera: Noctuidae)
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DOI：
10.1016/j.pestbp.2012.05.001
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发表时间：
2012-07-01
影响因子：
4.7
通讯作者：
Razaq, Muhammad
中科院分区：
文献类型：
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作者：
Abbas, Naeem;Shad, Sarfraz Ali;Razaq, Muhammad
吡虫啉已被用作控制棉花吸食性害虫的关键杀虫剂，而斜纹夜蛾在巴基斯坦也间接接触了该杀虫剂。为了评估抗性进化的风险和制定更好的抗性管理策略，在实验室用吡虫啉对田间采集的种群进行了选择。研究了斜纹夜蛾对吡虫啉抗性的适应成本、已实现遗传力和交叉抗性。经过14代吡虫啉的筛选，斜纹夜蛾对该杀虫剂的抗性提高了137.48倍。生物测定结果表明，该菌株对扑草胺有交叉抗性(RR 8.52)，对氯氰菊酯有少量交叉抗性(1.92)，对灭多威呈负交叉抗性(-0.19)。抗性品系的相对适合度为0.38，幼虫存活率、幼虫历期、雄蛹历期、发育历期、健康成虫羽化率、繁殖力、孵化率、幼虫和蛹历期均显著低于抗性品系。所选种群的幼虫平均相对生长率、种群内禀增长率和生物潜力都较低。斜纹夜蛾抗性品系对吡虫啉的抗性实现遗传力(h(2))为0.15。抗药性的发展可能要花费大量的抗药性种群的适合度。本研究为进一步了解吡虫啉抗性对斜纹夜蛾生理参数的影响以及制定抗性管理策略提供了有价值的信息。(C)2012 Elsevier Inc.保留所有权利。
Imidacloprid has been used as a key insecticide for controlling sucking insect pests of cotton, whereas Spodoptera litura also has been indirectly exposed to this insecticide in Pakistan. To evaluate the risk of resistance evolution and to develop a better resistance management strategy, a field collected population was selected with imidacloprid in the laboratory. Thereafter, fitness cost, realized heritability and cross resistance of imidacloprid resistance in S. litura were investigated. After 14 generations of selection with imidacloprid, S. litura developed a 137.48-fold resistance to the insecticide. Bioassay revealed that this strain showed cross-resistance to acetamiprid (RR 8.52) and a little to lamdacyhalothrin (1.92) but negative cross-resistance was found to methomyl (-0.19). The resistant strain had a relative fitness of 0.38, with substantially lower rates of larval survival, larval duration, male pupal duration, development time, emergence rate of healthy adults, fecundity, hatchability, and prolonged larval and pupal duration. Mean relative growth rate of the larvae, intrinsic rate of population increase, and biotic potential was lower for the selected populations. The estimated realized heritability (h(2)) of imidacloprid resistance was 0.15 in the resistant strain of S. litura. Development of the resistance may cost significant fitness for the resistant population. This study provided valuable information for further understanding the impact of imidacloprid resistance on physiological parameters of S. litura and for facilitating the development of resistance management strategies. (C) 2012 Elsevier Inc. All rights reserved.