Effective enrichment of prostate cancer stem cells from spheres in a suspension culture system
Effective enrichment of prostate cancer stem cells from spheres in a suspension culture system
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DOI：
10.1016/j.urolonc.2010.03.019
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发表时间：
2012-05-01
影响因子：
2.7
通讯作者：
Lin, Tianxin
中科院分区：
文献类型：
--
作者：
Fan, Xinlan;Liu, Shanying;Lin, Tianxin
背景：干细胞样前列腺癌细胞也称为前列腺癌干细胞（PrCSC）。这些罕见的细胞被认为是高度致瘤性的，并参与维持肿瘤稳态和介导肿瘤转移。用于分选PrCSC的方法主要基于分选具有标志物（CD133（+）/CD44（+））的细胞或侧群细胞。然而，CD 133（+）/CD 44（+）细胞或侧群细胞非常罕见，甚至无法检测到。方法：采用悬浮培养法富集PrCSCs。结果：PC3细胞在非贴壁悬浮培养中存活，并形成球形细胞。非贴壁球体形成细胞群中的CD44（+）/CD133（+）细胞百分比是贴壁PC 3细胞群中的18倍（分别为13.94%和0.77%）。在非粘附人群中，该侧人群增加至3.1%，但在粘附人群中未检测到。结论：悬浮培养可用于PrCSCs的富集。这种方法将有助于前列腺干细胞生物学研究，并促进前列腺癌新治疗药物的鉴定。(C)2012 Elsevier Inc. All rights reserved.
Background: Stem-like prostate cancer cells are also called prostate cancer stem cells (PrCSCs). These rare cells are supposed to be highly tumorigenic and to be involved in maintenance of tumor homeostasis and mediation of tumor metastasis. Methods for sorting PrCSCs are mainly based on sorting cells with the marker (CD133(+)/CD44(+)) or side population cells. However, CD133(+)/CD44(+) cells or side population cells are very rare or even undetectable. The scarcity of approaches for isolation and purification of PrCSCs is the main obstacle to studying PrCSCs.Methods: In the present study, suspension culture was used for enrichment of PrCSCs. And PrCSCs were verified by side population technology, drug sensitivity assays, and the molecular marker analysis of prostate cancer stem cell.Results: PC3 cells survived and formed spheres in nonadherent suspension culture. The percentage of CD44(+)/CD133(+) cells was 18-fold higher in the nonadherent sphere-forming cell population than in the adherent PC3 cell population (13.94% vs. 0.77%, respectively). This side population was increased to 3.1% in the nonadherent population but undetectable in adherent population. Resistance to cisplatin was higher in the nonadherent cells than adherent cells.Conclusion: Suspension culture can be used to enrich for PrCSCs. This approach will aid prostate stem cell biology research and facilitate identification of novel therapeutic agents for prostate cancer. (C) 2012 Elsevier Inc. All rights reserved.