The Bovine Metabolome
The Bovine Metabolome
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DOI：
10.3390/metabo10060233
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发表时间：
2020-06-01
期刊：
影响因子：
4.1
通讯作者：
Wishart, David S.
中科院分区：
文献类型：
--
作者：
Foroutan, Aidin;Fitzsimmons, Carolyn;Wishart, David S.
从动物健康的角度来看，对牛生物体液和组织中许多代谢物的典型或健康浓度范围知之甚少。在这里，我们描述了一个全面的，定量的代谢组学表征的六个牛的生物流体和组织，包括血清，瘤胃液，肝脏，胸最长肌（LT）肌肉，半膜肌（SM）肌肉和睾丸组织的结果。使用核磁共振（NMR）光谱，液相色谱-串联质谱（LC-MS/MS）和电感耦合等离子体质谱（ICP-MS），我们能够识别和定量这些生物液体/组织中的超过145种代谢物。将这些结果与我们团队先前对其他牛生物流体所做的工作以及先前发表的其他牛组织和生物流体的文献值相结合，我们能够生成五种不同牛生物流体和七种不同组织中2100种独特代谢物的定量参考浓度数据。这些实验数据与计算机辅助的基因组规模代谢物推断技术相结合，增加了另外48，628种独特的代谢物，这些代谢物在生物化学上预计将存在于牛组织或生物液体中。在本研究中共鉴定了51，801种独特的代谢物。关于这51，801种独特代谢物的详细信息已被放置在一个名为牛代谢组数据库的公开数据库中。
From an animal health perspective, relatively little is known about the typical or healthy ranges of concentrations for many metabolites in bovine biofluids and tissues. Here, we describe the results of a comprehensive, quantitative metabolomic characterization of six bovine biofluids and tissues, including serum, ruminal fluid, liver, Longissimus thoracis (LT) muscle, semimembranosus (SM) muscle, and testis tissues. Using nuclear magnetic resonance (NMR) spectroscopy, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and inductively coupled plasma-mass spectrometry (ICP-MS), we were able to identify and quantify more than 145 metabolites in each of these biofluids/tissues. Combining these results with previous work done by our team on other bovine biofluids, as well as previously published literature values for other bovine tissues and biofluids, we were able to generate quantitative reference concentration data for 2100 unique metabolites across five different bovine biofluids and seven different tissues. These experimental data were combined with computer-aided, genome-scale metabolite inference techniques to add another 48,628 unique metabolites that are biochemically expected to be in bovine tissues or biofluids. Altogether, 51,801 unique metabolites were identified in this study. Detailed information on these 51,801 unique metabolites has been placed in a publicly available database called the Bovine Metabolome Database.