Characterization and antioxidant activity of a polysaccharide extracted from Sarcandra glabra
Characterization and antioxidant activity of a polysaccharide extracted from Sarcandra glabra
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从草珊瑚中提取的多糖的表征和抗氧化活性
DOI：
10.1016/j.carbpol.2012.05.074
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发表时间：
2012-09-01
影响因子：
11.2
通讯作者：
Gao, Xiangdong
中科院分区：
文献类型：
--
作者：
Jin, Lei;Guan, Xin;Gao, Xiangdong
采用响应面法（RSM）优化了从大鲵地上部位提取多糖的工艺参数。结果表明，最佳提取条件为：水料比为30，提取温度为85℃，提取时间为3 h，得率为4.55%。在最佳条件下，SGP的实验产率为4.49 +/- 0.09%。采用DEAE-cellulose-52和sepphacryl S-400柱层析纯化得到多糖SGP-1。SGP-1在高效阻垢色谱（HPSEC）中呈单峰对称，平均分子量（Mw）估计为1.06 × 10(4) Da。由葡萄糖、半乳糖和甘露糖组成，经气相色谱法测定其比例为8.38:3.13:1。体外抗氧化实验表明，SGP-1对羟基、超氧阴离子、DPPH均有明显的抑制作用。ABTS自由基呈剂量依赖性。本研究表明SGP-1可以作为一种潜在的天然抗氧化剂。(C) 2012 Elsevier Ltd.版权所有。
Response surface methodology (RSM) was employed to optimize the parameters for polysaccharide extracted from the aerial parts of Sarcandra glabra (SGP). The optimum conditions were predicted as follows, ratio of water to raw material at 30, extracting temperature at 85 degrees C. extracting duration at 3 h, and yield was estimated at 4.55%. The experimental yield of SGP under the optimum conditions was 4.49 +/- 0.09%. A homogenous polysaccharide (SGP-1) was obtained by purification using DEAE-cellulose-52 and Sephacryl S-400 column chromatography. SGP-1 showed a single symmetrical peak in high performance size-exclusion chromatography (HPSEC) and the average molecular weight (Mw) was estimated to be 1.06 x 10(4) Da. It was composed of glucose, galactose, and mannose in a ratio of 8.38:3.13:1 determined by gas chromatography (GC). The in vitro antioxidant tests showed that SGP-1 has significant inhibition effects on hydroxyl, superoxide anion, DPPH. ABTS radicals in a dose-dependent manner. This study indicated that SGP-1 could be used as a potential natural antioxidant. (C) 2012 Elsevier Ltd. All rights reserved.