HETEROGENEITY OF EPSTEIN-BARR-VIRUS INFECTION IN ANGIOIMMUNOBLASTIC LYMPHADENOPATHY TYPE T-CELL LYMPHOMA
HETEROGENEITY OF EPSTEIN-BARR-VIRUS INFECTION IN ANGIOIMMUNOBLASTIC LYMPHADENOPATHY TYPE T-CELL LYMPHOMA
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DOI：
10.1111/j.1365-2559.1994.tb01376.x
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发表时间：
1994-12-01
期刊：
影响因子：
6.4
通讯作者：
AKAMATSU, M
中科院分区：
文献类型：
--
作者：
OHSHIMA, K;TAKEO, H;AKAMATSU, M
为了研究 Epstein-Barr 病毒 (EBV) 和血管免疫母细胞性淋巴结病与异常蛋白血症的关系，我们使用聚合酶链式反应 (PCR)、Southern 印迹、原位杂交和免疫组织化学分析对 5 名接受多次随访和活检的患者的淋巴结进行 DNA 分析。在疾病过程中，淋巴结结构减少，细胞异型性恶化，透明细胞数量增加。在受体基因的 DNA 分析中，克隆群体的数量有所增加。通过 PCR 或原位杂交在所有检查的节点中发现了 EBV 核酸序列。不同病例以及同一患者的整个病程中 EBV 阳性细胞的数量差异很大。 EBV 末端重复序列或淋巴细胞确定的膜抗原基因的分析显示了 EB 感染细胞的多克隆群体。 EBV阳性细胞具有中等到大尺寸的细胞核，特别是大细胞核的细胞表达EBV的潜在膜蛋白。这些大细胞的数量因病例而异。双标记免疫组织化学/原位杂交研究表明，大多数 EBV 阳性细胞表达 B 细胞抗原 (CD20)。 EBV 的存在似乎与免疫系统的选择性缺陷有关，而不是与血管免疫母细胞性淋巴结病的直接发病机制有关。
To investigate the relationship of Epstein-Barr virus (EBV) and angioimmunoblastic lymphadenopathy with dysproteinemia, we performed DNA analysis using the polymerase chain reaction (PCR), Southern blot, in situ hybridization, and immunohistochemical analysis of lymph nodes in five patients who were followed up and biopsied more than once. In the course of the disease, nodal architecture diminished, cellular atypia worsened, and clear cells increased in number. In the DNA analysis of the receptor genes, the clonal population increased in number. EBV nucleic acid sequences were found by either PCR or in situ hybridization in all examined nodes. The number of EBV-positive cells varied widely among the cases and throughout the course of the disease in the same patients. The analysis of EBV terminal repeats or lymphocyte-determined membrane antigen genes showed polyclonal populations of EB-infected cells. EBV-positive cells possessed intermediate- to large-sized nuclei, and the cells with large nuclei, especially, expressed latent membrane protein of EBV. These large cells varied in number among the cases. Double-labelling immunohistochemistry/in situ hybridization studies demonstrated that most of the EBV-positive cells expressed B-cell antigen (CD20). The presence of EBV seems to be associated with the selective defects of the immune system, rather than with the direct pathogenesis of angioimmunoblastic lymphadenopathy.