CHARACTERIZATION OF HUMAN AND MOUSE CARTILAGE OLIGOMERIC MATRIX PROTEIN
CHARACTERIZATION OF HUMAN AND MOUSE CARTILAGE OLIGOMERIC MATRIX PROTEIN
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DOI：
10.1006/geno.1994.1649
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发表时间：
1994-12-01
期刊：
影响因子：
4.4
通讯作者：
LAWLER, J
中科院分区：
文献类型：
--
作者：
NEWTON, G;WEREMOWICZ, S;LAWLER, J
软骨寡聚基质蛋白是一种524,000 Da的蛋白，在软骨细胞的区域基质中高水平表达。大鼠和牛的COMP序列表明它是凝血酶反应蛋白基因家族的成员。在本研究中，我们克隆并测序了人COMP。用递进序列比对和两种简约算法进行的系统发育分析表明，comp基因和凝血酶反应蛋白-3和-4基因的前体是发生在7.5亿年前的基因复制所产生的。利用种间回交定位系统将小鼠COMP基因定位于小鼠8号染色体的中心区域。体细胞杂交DNA定位系统的Southern杂交和与人中期染色体的原位杂交表明，人COMP基因位于19号染色体p13.1带。这些数据证实并扩展了人和小鼠染色体同源性的已知区域，证实了COMP与凝血酶原蛋白-1、-2、-3和-4一样存在于人和小鼠的基因组中。(C)1994年学术出版社。
Cartilage oligomeric matrix protein (COMP) is a 524,000-Da protein that is expressed at high levels in the territorial matrix of chondrocytes. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. In this study, we have cloned and sequenced human COMP. Phylogenetic analysis using progressive sequence alignment and two parsimony-based algorithms indicates that the COMP gene and a precursor of the thrombospondin-3 and -4 genes were produced by a gene duplication that occurred 750 million years ago. An interspecific backcross mapping panel has been used to map the murine COMP gene to the central region of mouse chromosome 8. Southern blot analysis of a somatic cell hybrid DNA panel and in situ hybridization to human metaphase chromosomes indicate that the human COMP gene is located on chromosome 19 in band p13.1, These data confirm and extend the known regions of homology between human and mouse chromosomes and establish that COMP, like thrombospondin-1, -2, -3, and -4, is present in the human and mouse genomes. (C) 1994 Academic Press, Inc.