Physiological and immunological studies with desamidogastrin.
Physiological and immunological studies with desamidogastrin.
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去酰胺胃泌素的生理学和免疫学研究。
DOI：
10.1016/s0016-5085(72)80038-6
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发表时间：
1972
期刊：
影响因子：
29.4
通讯作者：
Henry F. Thomas
中科院分区：
文献类型：
--
作者：
J. Mcguigan;Henry F. Thomas
胃泌素分子的羧基末端四肽酰胺已被证明在失去其末端酰胺基时生理上没有活性。本研究测定了非酰胺化人胃泌素I(Desamidogastin)的生理活性。分别以0.1g/kg、1.0g/h和10g/μ/h的剂量持续静脉滴注去氨基胃泌素给海登海因胶囊犬。与人类胃泌素I相反，我们发现去氨基胃泌素对海登海因胃袋的产酸没有明显的刺激作用。此外，还评估了去氨基胃泌素与抗人胃泌素I抗体的免疫反应性。抗人胃泌素I抗体是用人胃泌素I与牛血清白蛋白共价偶联的2~17位残基反复免疫兔产生的抗体。除去胃泌素羧基末端的酰胺基团后，人胃泌素I分子对这些抗体的免疫反应性丧失了99%以上。
The carboxyl-terminal tetrapeptide amide of the gastrin molecule has been shown to be physiologically inactive when deprived of its terminal amide group. In this study, the physiological activity of nonamidated human gastrin I (desamidogastrin) was examined. Desamidogastrin, in doses of 0.1, 1.0, and 10 μg per kg per hr, was administered by constant intravenous infusion to dogs prepared with Heidenhain pouches. In contrast to human gastrin I, desamidogastrin was found without measurable effect in stimulating acid output from the Heidenhain pouches. In addition, the immunological reactivity of desamidogastrin with antibodies to human gastrin I was assessed. Antibodies to human gastri n I utilized were those produced by repeated immunization of rabbits with human gastrin I, residues 2 to 17, covalently conjugated to bovine serum albumin. In addition to the absence of demonstrable physiological effect in stimulating acid secretion, when the gastrin carboxyl-terminal amide group was removed, greater than 99% of the immunological reactivity of human gastrin I molecules with these antibodies to gastrin was lost.