Metabolic engineering of Yarrowia lipolytica for improving squalene production.
Metabolic engineering of Yarrowia lipolytica for improving squalene production.
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DOI：
10.1016/j.biortech.2020.124652
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发表时间：
2021-01
影响因子：
11.4
通讯作者：
Wenyan Tang;Dong-Ping Wang;Yun Tian;Xiao Fan;Chong Wang;Xiangyang Lu;Peiwang Li;Xiaojun Ji;Huhu Liu
中科院分区：
文献类型：
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作者：
Wenyan Tang;Dong-Ping Wang;Yun Tian;Xiao Fan;Chong Wang;Xiangyang Lu;Peiwang Li;Xiaojun Ji;Huhu Liu
The aim of this present research is to enhance the squalene production inYarrowia lipolyticausing pathway engineering and bioprocess engineering. Firstly, to improve the production of squalene, the endogenous HMG-CoA reductase (HMG1) was overexpressed inY. lipolyticato yield 208.88 mg/L squalene. Secondly, the HMG1 and diacylglycerol acyltranferase (DGA1) were co-overexpressed, the derived recombinantY. lipolyticaSQ-1 strain produced 439.14 mg/L of squalene. Thirdly, by optimizing the fermentation medium, the improved titer of squalene with 514.34 mg/L was obtained by the engineered strain SQ-1 grown on YPD-80 medium. Finally, by optimizing the addition concentrations of acetate, citrate and terbinafine, the 731.18 mg/L squalene was produced in the engineered strain SQ-1 with the addition of 0.5 mg/L terbinafine. This work describes the highest reported squalene titer inY. lipolyticato date. This study will provide the foundation for further engineeringY. lipolyticacapable of cost-efficiently producing squalene.
