Streptococcus gordonii promotes rapid differentiation of monocytes into dendritic cells through interaction with the sialic acid-binding adhesin
Streptococcus gordonii promotes rapid differentiation of monocytes into dendritic cells through interaction with the sialic acid-binding adhesin
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DOI：
10.1007/s10266-011-0044-z
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发表时间：
2012-07-01
期刊：
影响因子：
2.5
通讯作者：
Konishi, Kiyoshi
中科院分区：
文献类型：
--
作者：
Urano-Tashiro, Yumiko;Yajima, Ayako;Konishi, Kiyoshi
感染性心内膜炎常归因于口腔链球菌。虽然致病机制尚未完全了解，链球菌和吞噬细胞之间的相互作用被认为是重要的感染性心内膜炎。在这项研究中，HL-60细胞衍生的单核细胞的特点与戈登链球菌DL 1的相互作用。单核细胞暴露于S.戈登氏菌DL 1诱导树突状细胞（DC）标志物CD 83、CD 1a、CD 86和白细胞介素-12的上调，而单核细胞标志物PU.1和MafB（其通常以低水平存在于成熟DC中）下调。HL-60来源的单核细胞与S.在无细胞因子释放的情况下，gordonii DL 1对DC分化具有指导意义。此外，S. gordonii和缺乏唾液酸结合活性的hsa突变体都能够诱导HL-60细胞分化。总而言之，这些数据表明用S. gordonii DL 1通过与细菌表面受体Hsa相互作用迅速经历单核细胞向DC分化，并且这种反应可能是口腔链球菌感染性心内膜炎的初始步骤。
Infective endocarditis is frequently attributed to oral streptococci. Although the pathogenetic mechanisms are not well understood, interaction between streptococci and phagocytes is thought to be important for infective endocarditis. In this study, HL-60 cell-derived monocytes were characterized following interaction with Streptococcus gordonii DL1. Exposure of monocytes to S. gordonii DL1 induced up-regulation of the dendritic cell (DC) markers CD83, CD1a, CD86, and interleukin-12, while monocyte markers PU.1 and MafB, which are typically present at low levels in mature DCs, were down-regulated. Interaction of HL-60-derived monocytes with S. gordonii DL1 was instructive for DC differentiation in the absence of released cytokines. Furthermore, neither the filtered culture medium of S. gordonii nor the hsa mutant, deficient in sialic acid-binding activity, was able to induce the differentiation of HL-60 cells. Taken together, these data suggest that monocytes stimulated with S. gordonii DL1 rapidly undergo monocyte-to-DC differentiation through interaction with the bacterial surface receptor Hsa and that this response may be the initial step in infective endocarditis by oral streptococci.