Immune electron microscopy of hepatitis δ-antigen in hepatocytes
Immune electron microscopy of hepatitis δ-antigen in hepatocytes
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肝细胞δ型肝炎抗原的免疫电镜观察
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
Desmet Vj
中科院分区：
文献类型：
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作者：
T. Kojima;F. Callea;J. Desmyter;Desmet Vj
对 7 名慢性乙型肝炎伴 δ 感染患者的肝活检进行了 δ 型肝炎 δ 抗原（HDAg）的免疫电子显微镜检查。 HDAg 主要存在于细胞核中，但在一些分散的肝细胞中，它也存在于细胞质中，或者与核糖体相关，或者在透明质中。 HDAg 与核糖体的结合可能是同一核糖体上 δ-信使 RNA 翻译的结果。在细胞核中，δ抗原以不规则颗粒结构形式存在于染色质区域，并且存在20至30 nm颗粒结构聚集体的染色，类似于首先在非甲非乙型肝炎中描述的那些。然而，HDAg 阴性细胞核中也存在类似的颗粒结构。血液中描述的 δ 颗粒无法在肝细胞中识别。
Immune electron microscopic examination of hepatitis delta delta-antigen (HDAg) was carried out on liver biopsies from seven patients with chronic type B hepatitis and delta-infection. HDAg was mainly found in the nucleus, but in some scattered hepatocytes it was found in the cytoplasm also, either in association with ribosomes or in the hyaloplasm. The association of HDAg with ribosomes may be the result of translation from delta-messenger RNA on the same ribosomes. In the nucleus, delta-antigen was present in the chromatin area as irregular granular structures, and there was staining of aggregates of 20 to 30-nm particulate structures, similar to those first described in non-A, non-B hepatitis. However, similar particulate structures were also present in HDAg negative nuclei. The delta-particles which have been described in blood could not be identified in hepatocytes.