Antioxidant activity of green tea and its catechins in a fish meat model system

Antioxidant activity of green tea and its catechins in a fish meat model system
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DOI:
10.1021/jf9706134
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发表时间:
1997-11-01
影响因子:
6.1
通讯作者:
Shahidi, F
Shahidi, F
中科院分区:
农林科学1区
文献类型:
--
作者:
He, YH;Shahidi, F

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研究了磨碎的绿色茶(GGT)和商品茶提取物,即Polyphenon 25(P-25)、Polyphenon 30(P-30)、Polyphenon 60(P-60)和Nikken Polyphenon 60(NPP-60),以及实验室规模制备的绿色茶提取物(GTE)和纯茶儿茶素,即(-)表儿茶素(EC)、(-)-表没食子儿茶素(EGG)、评价了(-)-表儿茶素没食子酸酯(ECG)和(-)-表没食子儿茶素没食子酸酯(EGCG)在鱼肉模型系统中的作用。将所得结果与含有常用抗氧化剂如α-生育酚、丁基化羟基甲苯(BHT)、丁基化羟基茴香醚(BHA)和叔丁基对苯二酚(TBH&)的样品的结果进行比较。将鲭鱼的磨碎的白色肌肉(模型系统)在75 ℃下烹饪并在4 ℃下储存7天。通过测量2-硫代巴比妥酸反应性物质和样品的选定和/或总挥发物含量的变化来监测氧化的进展。与含有α-生育酚、BHT、BHA和TBHQ的样品相比,用GGT叶、茶提取物和纯儿茶素处理的样品显示出优异的氧化稳定性。儿茶素对鱼肉的抗氧化作用由大到小依次为EGCG接近ECG > EGC远大于EC。然而,EGCG比TBHQ更有效(p < 0.05),如所研究的系统中的总挥发物和丙醛含量所反映的。
The antioxidant activity of ground green tea (GGT) and commercial tea extracts, namely Polyphenon 25 (P-25), Polyphenon 30 (P-30), Polyphenon 60 (P-60), and Nikken Polyphenon 60 (NPP-60), as well as green tea extracts (GTE) prepared on laboratory scale and pure tea catechins, namely, (-)epicatechin (EC), (-)-epigallocatechin (EGG), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG), in a fish meat model system was evaluated. Results so obtained were compared with those of samples containing commonly used antioxidants Such as alpha-tocopherol, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and tert-butylhydroquinone (TBH&). The ground white muscle of mackerel (model system) was cooked at 75 degrees C and stored at 4 degrees C for 7 days. Progression of oxidation was monitored by measuring changes in the 2-thiobarbituric acid-reactive substances and selected and/or total volatile contents of samples. The samples treated with GGT leaves, tea extracts, and pure catechins showed excellent oxidative stability as compared with samples that contained alpha-tocopherol, BHT, BHA, and TBHQ. The potency of catechins in the prevention of oxidation in fish meat was in the decreasing order EGCG approximate to ECG > EGC much greater than EC. However, EGCG was more effective (p < 0.05) than TBHQ, as reflected in total volatile and propanal contents in the system studied.